带血管鼠膝关节冷冻保存研究  

An Experimental study of cryopreservation of rat knee joint with vessels

作　　者：周建生[1] 郑圣鼐 潘功平[1] 胡妆麒 肖玉周[1] 刘振华[1] 王霄光 马强[1] 

机构地区：[1]蚌埠市蚌埠医学院附属医院骨科,233004

出　　处：《中国临床解剖学杂志》2000年第2期172-173,176,共3页Chinese Journal of Clinical Anatomy

摘　　要：目的 :探讨 15 %DMSO浸泡时间对冷冻保存带血管鼠膝关节生物活性、超微结构的影响。方法 :40只SD大鼠带血管膝关节分别在 4℃ 15 %DMSO中浸泡 0 .5、1、1.5、2h ,按两步冷冻保存 3 0天后 ,电镜观察超微结构变化 ,软骨细胞台盼蓝染色及软骨细胞3 H TdR掺入率测定软骨细胞存活率及细胞活性。结果 :电镜下 1h组、1.5h组血管内皮细胞改变轻微 ;0 .5h组、2h组内皮细胞超微结构损伤严重。 1.5h组软骨细胞存活率及细胞活性高于其余冷冻组 (P <0 .0 1)。结论 :带血管鼠膝关节在 4℃ 15 %DMSO中浸泡 1.5h后两步冷冻保存效果良好。Objective:To explore the effect of different exposure time in 15% DMSO on the biological activity and ultrastructure of cryopreserved rat knee joint with vessels.Methods:Forty rat knee joint pedicled with femoral vessels were immersed into 15% DMSO at 4℃ for 0.5 hour,1 hour,1.5 hours,and 2 hours respectively.After they were cryoperserved by a two step schedule in 30 days,the ultrastructure were observed under TEM,and the chondrocyte survival rates and viability were evaluated by trypan blue staining and H 3 TdR incorporation rate respectively.Results:The ultrastructure of endothelial cell changed mildly in 1h and 1.5h groups,but serious damaged in 0.5h and 2h groups.Chondrocyte survival rates and viability in 1.5h group were significantly higher than that of other groups (P<0.01).Conclusion:Good effect could be received by expose the rat knee joint in 15% DMSO for 1.5 hours at 4℃ before it preserved by two step cryopreservation.

关 键 词：膝关节 带血管关节 冷冻保存 SD大鼠 

分 类 号：R684[医药卫生—骨科学] R318.52[医药卫生—外科学]