薄层扫描法测定香科属植物中4种新克罗烷型二萜的含量  

DETERMINATION OF FOUR NEO CLERODANE DITERPENOIDS OF TEUCRIUM SPECIES BY HPTLC SCANNING

作　　者：王磊[1] 罗淑荣[1] 李广义[1] 

机构地区：[1]中国医学科学院

出　　处：《药学学报》2000年第4期294-296,共3页Acta Pharmaceutica Sinica

摘　　要：目的 :用薄层扫描法测定香科属植物中 4种新克罗烷型二萜 :teucvidin (I) ,19 acetyl teuspinin (II) ,teuscorodonin (III) ,teuperninA (IV)。 方法 :样品经丙酮超声提取后 ,用XAD 2型大孔树脂去除色素 ;在高效硅胶薄层板上点样后展开 ,展开剂为乙酸乙酯—正己烷 (6∶5 )。所得斑点用Ehrlich试剂显色 ,扫描测定。结果 :各新克罗烷型二萜的线性范围在 0 6 3～ 2 18μg ,回收率为 92 4%～ 97 8% ,应用本法测定了香科属植物铁轴草、庐山香科丙酮浸膏中新克罗烷型二萜的含量。结论 :本法简便易行 ,分析速度快 ,重现性好 ,可用于香科属植物中新克罗烷型二萜的测定。为开发资源 ,评价药物质量 。AIM: To establish a TLC scanning method for the separation and determination of the four neo clerodane diterpenoids: teucvidin (I), 19 acetyl teuspinin (II), teuscorodonin (III) and teupernin A (IV) in T.quadrifarium and the acetone extract of T.pernyi from Teucrium species. METHODS: The sample was extracted with supersonic waves with acetone. Then XAD 2 macroporous resin was used to remove the pigment. EtOAc — n hexane (6∶5) was used as developing solvent on the HPTLC silica gel plate. After that, the spots were identified by Ehrlich reagent and then determined with a Shimadzu CS 930 TLC scanner. RESULTS: The calibration curves were linear in the range of 0 63～2 18 μg for the four neo clerodane diterpenoids and the recoveries were 92 4%～97 8%. The method had been applied to the analysis of two plants. CONCLUSION: The method is simple, feasible, rapid and reproducible. It offered a scientific basis for seeking and utilizing botanic resources, and evaluating the quality of herb medicines.

关 键 词：薄层扫描法 得料属 新克罗烷型二萜 

分 类 号：R284.1[医药卫生—中药学]