库仑滴定法测定血清胆固醇的含量(英文)  

DETERMINATION OF CHOLESTEROL IN HUMAN SERUM BY COULOMETRIC TITRATION

作　　者：文志明[1] 刘爱茹[1] 徐礼燊 

机构地区：[1]中国医学科学院

出　　处：《药学学报》2000年第4期297-300,共4页Acta Pharmaceutica Sinica

摘　　要：目的 :研究库仑滴定法测定血清胆固醇含量的分析方法。方法 :以 1mol·L- 1KBr—冰醋酸 (1∶3)为电解液 ,在阳极电生溴与胆固醇发生加成反应测定胆固醇含量。结果 :胆固醇与溴反应 ,其反应电子数n为 2。胆固醇的加入量为 0 115～ 0 4 59mg时 ,其回收率为 93 9%～ 10 5% ,平均回收率为 10 0 3% ,方法的RSD <1 5%。应用本方法对人血清样品中胆固醇含量进行了测定 ,并与酶法测定结果进行了对比。结论 :本法简便、快速、准确 ,可用于血清胆固醇含量的测定。AIM: To study the determination method of cholesterol in human serum by Coulometric titration. METHODS: The titration was carried out in an electrolytic solution containing 1 mol·L -1 potassium bromide and glacial acetic acid (1∶3). Bromine was electrogenerated at the anode and cholesterol reacted with bromine with a value of reactional electrons ( n ) as 2. RESULTS: The ranges of recovery for 0 115 mg to 0 459 mg of cholesterol were from 93 9% to 105%, and the average recovery was 100 3%. The relative standard deviation in all applied analysis was less than 1 5%. The accuracy of the method was also assessed in comparison with the results obtained by the enzymatic method used clinically. CONCLUSION: The method proposed is simple, rapid and accurate, and is suitable for the determination of cholesterol in human serum samples.

关 键 词：胆固醇 血清诊断 库仑滴定法 

分 类 号：R446.112[医药卫生—诊断学]