醋柳总黄酮对兔血管紧张素转换酶的抑制作用  

Inhibitory effect of total flavones of hippophae rhamnoides L on angiotensin converting enzyme from rabbit

作　　者：朱福 章茂顺[2] 王家良[2] 符宗胤[2] 

机构地区：[1]上海市南汇中心医院心内科,上海201300 [2]华西医科大学附属第一医院心内科,成都610041

出　　处：《中国临床药学杂志》2000年第2期95-98,共4页Chinese Journal of Clinical Pharmacy

摘　　要：目的 :探讨醋柳总黄酮 (TFH)及其单体降压及心血管综合保护作用机制。方法 :紫外分光光度法和放射免疫法检测TFH、槲皮素 (Que)、异鼠李素 (Isor)对培养的兔主动脉平滑肌细胞 (ASMC)及兔血浆血管紧张素转换酶 (ACE)活性及血管紧张素 (Ang )生成的影响 ,用卡托普利 (Cap)作对照。结果 :TFH、Que、Isor对 ASMC ACE活性及 Ang 生成有显著持久的抑制作用 ,对血浆 ACE活性及 Ang 生成也有短暂抑制作用 ;TFH、Que、Isor对 ASMC、ACE活性和 Ang 生成抑制作用明显强于兔血浆 ,持续时间较血浆长。结论 :TFH及其单体对 ACE活性及 Ang 生成的抑制作用 ,特别是细胞内作用 ,可能是其降压及心血管综合保护作用的重要机制之一。AIM: To investigate the mechanism of the antihypertention and the cardiovascular comprehensive protecting effect of total flavones of hippophae rhamnoides L (TFH) and its essential monomers, namely quercetin (Que) and isorhametin (Isor). METHODS: The ultraviolet photospectrography assay and the radioimmunoassay were used to measure the effects of TFH, Que and Isor on the angiotensin converting enzyme (ACE) activity and the AngⅡ formation of cultured aortic smooth muscle cells (ASMCs) and plasma from rabbit. Captopril (Cap) was used as control. RESULTS: (1) TFH, Que and Isor had remarkable and persistent inhibitory effect on the ACE activity and the AngⅡ formation of ASMCs, and had transient inhibitory effect on those of plasma. (2) TFH, Que and Isor had more powerful inhibitory effect and longer inhibitory time on the ACE activity and the AngⅡ formation of ASMCs than on that of plasma. CONCLUSION: The inhibitory effect of TFH and its essential monomers on the ACE activity and the AngⅡ formation, especially on the intracellular effect, is one of the probably important mechanisms of antihypertension and comprehensive protecting effect on cardiovascular system damage.

关 键 词：醋柳总黄酮 降压 兔 血管紧张素转换酶 抑制作用 

分 类 号：R96[医药卫生—药理学]