转Cry1Ac抗虫棉对土壤虫生真菌群落的影响  

Effects of transgenic Cry1Ac cotton on entomopathogenic fungi in soil

作　　者：谢明[1] 张艳军[1] 吴刚[1] 

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室/农业部生物防治重点开放实验室,北京100193

出　　处：《应用昆虫学报》2012年第4期946-950,共5页Chinese Journal of Applied Entomology

基　　金：转基因专项(2008ZX08012-004;2009ZX08011-028B)

摘　　要：利用大蜡螟Galleria mellonella L.诱菌法对转Cry1Ac抗虫棉GK-12及其亲本棉花SM-3的根系区域土壤的虫生真菌种类和数量进行监测,评价转Cry1Ac基因对棉田土壤虫生真菌的影响。实验结果显示:检测到的虫生真菌种类,在转基因棉GK-12及其亲本棉SM-3之间无显著差异,均为球孢白僵菌(Beauveria bassiana)和金龟子绿僵菌(Metarhizium anisopliae);在棉花开花初期、盛花期、结铃期或吐絮期,土壤虫生真菌数量在抗虫棉GK-12和亲本SM-3之间存在显著的差异,其他生育期无显著差异;在距棉花主根不同距离(5 cm\15 cm\25 cm)的区域,土壤虫生真菌数量和种类在抗虫棉GK-12和亲本SM-3之间无显著差异。The species diversity and amount of soil fungi around the roots of the transgenic GK-12 cotton variety containing the Cry1Ac gene was compared to that of the non-transgenic SM-3 variety. There was no significant difference in soil fungal species between GK-12 and SM-3 plants, with the fungi Beauveria basslana and Metarhizium anisopliae found in the root area of both varieties. There were, however, significant differences in the soil fungal species associated with these two varieties during the boll forming and boll opening periods. There were no differences in species or quantity of fungi found at different distances （5 cm, 15 cm and 25 cm） from the roots of these cotton varieties. These results indicate that the presence of the Cry1Ac gene had no significant effect on fungal species and quantity.

关 键 词：转基因棉花 虫生真菌 虫诱集 球孢白僵菌 金龟子绿僵菌 

分 类 号：S562[农业科学—作物学]