血红素氧化酶-1治疗放射性皮肤损伤的疗效观察  

Effect of heme oxygenase-1 on radiation-induced skin injury

作　　者：宋传军[1] 孟星君[2] 谢玲[1] 陈青[1] 周俊东[1] 张舒羽[3] 吴锦昌[1] 

机构地区：[1]南京医科大学附属苏州医院放疗科,苏州215001 [2]南京医科大学附属苏州医院肿瘤诊断与治疗重点实验室 [3]苏州大学放射医学与防护学院

出　　处：《中华放射医学与防护杂志》2012年第3期230-232,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（81102078）

摘　　要：目的探讨介导血红素氧化酶-1（heineoxygenase，HO．1）的腺病毒在治疗急性放射性皮肤损伤中的作用。方法选取33只雄性sD大鼠，电脑数字表法随机分为3组，磷酸盐缓冲液（PBS）对照组、介导绿色荧光蛋白基因的腺病毒（Ad-EGFP）组及介导HO．1基因的腺病毒（Ad-HO．1）组，每组11只。每组取3只大鼠，用于检测腺病毒目的基因的表达。用直线加速器产生的40Gy负电子线照射各组余下大鼠臀部皮肤。照射后立即分别皮下注射PBS、Ad．EGFP或Ad．HO-1。采用半定量方法对皮肤损伤评分，每周评定2次，观察7周。结果注射Ad-HO．1的皮肤真皮层内见HO-1表达强阳性。照射14d后，Ad-HO-1组皮肤损伤明显低于PBS对照组和Ad-EGFP组（q=0．000-0．030，P〈0．05）。结论HO-1能显著减轻急性放射性皮肤损伤，表明Ad．HO-1可以用于治疗放射性皮肤损伤。Objective To investigate the effect of heme oxygenase-1 （HO-1） on the acute radiation-induced skin injury by gene transfer. Methods Thirty-three male SD rats were randomly divided into three groups as PBS-injected group, Ad-EGFP-injected group and Ad-HO-l-injected group （ n = 11 ）. In each group, three rats were used for determining the expression of target gene and the other rats were irradiated on the buttock skin with 40 Gy electron beam generated by a linear accelerator. Immediately after irradiation, rats were administered with a subcutaneous injection of PBS, Ad-EGFP or Ad-HO-1, respectively. Subsequently, the skin reactions were measured twice a week using the semi-quantitative skin injury scale. Results The strong positive expression of HO-I was observed in subcutaneous dermal tissue after injection of Ad-HO-1. Compared to the PBS-injected group or the Ad-EGFP-injected group, a significant mitigation of skin injury was observed in Ad-HO-1-injected mice 14 d after irradiation （ q = 0. 000 - 0. 030, P 〈 0. 05 ）. Conclusions HO-1 could significantly mitigate radiation-induced acute skin injury and Ad-HO-1 could be used to treat radiation-induced skin injury.

关 键 词：血红素氧化酶 腺病毒 放射性皮肤损伤 基因治疗 

分 类 号：R818[医药卫生—放射医学]