高低剂量X射线照射正常人淋巴母细胞基因表达转录谱的研究  

Gene transcriptional profiles in human lymphoblastoid cells with low and high doses of irradiation

作　　者：李坤[1] 刘伟[1] 侯殿俊[1] 乔建伟[1] 马娅[1] 贾喜明[1] 李洁清[1] 

机构地区：[1]山东省医学科学院放射医学研究所,济南250062

出　　处：《中华放射医学与防护杂志》2012年第3期249-254,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：山东省自然科学基金（ZR2010CM045）;山东省医药卫生科技发展计划（2009HZ078）

摘　　要：目的比较高低剂量X射线照射对基因表达影响的差异性，探讨不同剂量辐射生物效应的作用机制。方法采用包含有45033个基因的NimbleGen12X135K芯片分析0．1和5Gy照射正常人淋巴母细胞AHH-1培养6h后基因表达谱的改变，以差异为2倍以上且两组实验结果一致的标准确定为有效差异表达基因。用real-timePCR和Westernblot方法验证了共同表达基因PERP的表达。结果0．1Gy照射后，有760个基因表达上调，1222个基因表达下调；5Gy照射后，上调和下调基因分别是457和744个。在两个剂量照射条件下共同表达上调的基因有55个，同时下调的基因有339个。低剂量组差异表达基因主要参与细胞信号转导、DNA损伤反应等过程，高剂量组主要参与凋亡、细胞增殖分化等过程。real-timePCR和Westernblot方法验证了PERP的表达与芯片结果相-致，均表达下调。结论高低剂量照射后基因表达改变存在质和量的差异，可更好地阐释高低剂量电离辐射生物学效应的作用机制。Objective To compare the gene expression difference between 0. 1 and 5 Gy X-ray irradiated ceils, and to explore its possible mechanism. Methods A cDNA microarray corresponding to 45033 human genes was used to analyze the transcriptional profiles of normal human lymphoblastoid AHH- 1 cells at 4 h after 0.1 or 5 Gy irradiation. The genes with a fold change ≥ 2.0 were identified as the differentially expressed genes, real-time PCR and Western blot were used to confirm the expression of PERP. Results The microarray assay showed that there were 760 up-regulated genes and 1222 down- regulated genes in the cells at 0. 1 Gy, while there were 744 genes down-regulated and 457 genes up- regulated in the cells at 5 Gy. In addition, 55 genes were commonly up-regulated and 339 genes commonly down-regulated at 0. 1 and 5 Gy. The predominant biological processes of the differential genes responding to low-dose radiation include cell-cell signaling transduction and DNA damage response, and the altered genes after 5 Gy irradiation were related to cell proliferation, differentiation, and apoptosis. Moreover, the expression of PERP gene was down regulated, which was consistent with the data of microarray assay. Conclusions The quantitative and qualitative differences in the gene expressions may contribute to the diversed biological effects induced by low or high doses of ionizing radiation.

关 键 词：电离辐射 基因芯片 剂量 GO分析 

分 类 号：R142[医药卫生—公共卫生与预防医学]