肺炎支原体的三种检测方法的比较  

A comparison of three methods of detecting Mycoplasma pneumonia

作　　者：蔡利红[1] 季伟[1] 孙慧明[1] 李蓓荃[1] 严永东[1] 黄莉[1] 陈正荣[1] 闻芳[1] 邵雪军[1] 朱宏[1] 

机构地区：[1]苏州大学附属儿童医院呼吸病科,江苏省215003

出　　处：《江苏医药》2012年第13期1532-1534,共3页Jiangsu Medical Journal

基　　金：苏州社会发展科技计划项目(SS0702)

摘　　要：目的比较肺炎支原体(MP)的三种检测方法在呼吸道感染患儿不同年龄、病程中的应用价值。方法 193例呼吸道感染患儿,均行MP咽拭子快速培养、血清酶联免疫吸附试验MP-IgM半定量检测和痰MP-DNA-PCR检测,对检查结果进行对照分析。结果 193例患儿中,MP咽拭子快速培养和血清MP-IgM半定量检测检出MP阳性各47例(MP阳性率24.35%),痰MP-DNA-PCR法检出MP阳性8例(MP阳性率4.15%%)。咽拭子快速培养法和痰MP-DNA-PCR法检测不同年龄段患儿MP感染的阳性率无差异;年龄越大,血清MP-IgM检测法检测阳性率越高。三种方法检测不同病程阶段MP感染阳性率均无差别。结论联合采用上述三种方法可以提高MP感染的检出率。Objective To compare the clinical values of three methods of detecting Mycoplasma pneumonia（MP） infection. Methods The MAP in 193 children with respiratory tract infection was examined by three methods of MP rapid culture,MP-IgM antibody in serum and PCR. Results The positive rate of MP was higher detected by rapid culture（24. 35%） and by serology（24. 35%） than that by PCR（4.15%）. The positive rate of MP reported by rapid culture was not different from that by PCR among different age groups, which by serology was increased as age increased. The positive rate of MP reported by three methods was not significantly different among different stages of MP infection. Conclusion Combined use of rapid culture, serology and PCR can improve the detection rate of MP infection in children.

关 键 词：肺炎支原体 酶联免疫吸附检验 聚合酶链反应 

分 类 号：R725[医药卫生—儿科]