FEPAS牛肉单核细胞增生性李斯特菌几种检验方法比较  

Comparison of Several Methods for Detection of FEPAS Beef Listeria Monocytogenes

作　　者：孔义华[1,2] 焦彦朝 刘杰麟[2] 高博 罗阿东 曹云恒 

机构地区：[1]贵州省出入境检验检疫局技术中心,贵州贵阳550002 [2]贵阳医学院医学检验系,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第3期238-241,262,共5页Journal of Guiyang Medical College

基　　金：国家质检总局科技计划项目(2009IK165);贵州省高层次人才科研条件特助经费项目(TZJF-2007年-18号)

摘　　要：目的:探讨在食品检验中单核细胞增生性李斯特菌的检验方法。方法:采用常规培养法、全自动酶联免疫荧光分析仪筛选法、基因探针化学发光检测法、实时荧光定量PCR检测法对英国食品检验能力评价体系(FEPAS)牛肉单核细胞增生性李斯特菌水平测试提供的二份盲样(M164d02A和M164d02B)进行检测。结果:4种检测方法检测结果一致,样本M164d02A检出单核细胞增生性李斯特菌、样本M164d02B未检出单核细胞增生性李斯特菌,而实时荧光定量PCR检测法灵敏度较高,检测周期较短、特异性较高。结论:实时荧光定量PCR检测法在单核细胞增生性李斯特菌的检测中具有灵敏度高、检测时间短、特异性高等特点。Objective： To compare several methods for detection of the Listeria monocytogenes in the food. Methods： Four different methods including routine culture method, full-automatic enzyme- linked immunoassay screening method by fluorescence analyzer, gene probe chemiluminescence meth- od （AccuProbe）, and real-time fluorescent quantitative PCR method were used to test two blind Liste- ria monocytogene samples （M164dO2A and M164d02B） provided by England food examination per- formance assessment scheme （FEPAS）, and sensitivity, detection time and specificity among the 4 methods were compared. Results： The four testing methods showed the same results that Listeria monocytogenes were detected in sample M164d02A but not detected in sample M164d02B, while Real- time quantitative fluorescence PCR detection method was more sensitive with shorter detection time and higher specificity. Conclusions： Real-time quantitative fluorescence PCR detection method is sensitive with short detection time and high specificity for detection of Listeria monocytogenes.

关 键 词：利斯特菌 单核细胞增生 核酸探针 实时荧光定量PCR 检疫 

分 类 号：R372[医药卫生—病原生物学] R378.99[医药卫生—基础医学]