高效液相色谱测定开喉剑喷雾剂中岩白菜素的含量  

Determination of Bergenin Content in Kaihoujian Spray with High Performance Liquid Chromatography

作　　者：王珍珍[1] 张景照[1] 许乾丽[2] 茅向军[2] 郑丽会[3] 夏玉吉[3] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州省食品药品检验所,贵州贵阳550004 [3]贵州师范大学,贵州贵阳550001

出　　处：《贵阳医学院学报》2012年第3期267-269,共3页Journal of Guiyang Medical College

摘　　要：目的:建立开喉剑喷雾剂中八爪金龙主要成分岩白菜素含量的测定方法。方法:采用高效液相色谱法,色谱柱为Hypersil ODS2-C18柱(5μm,200 mm×4.6 mm),流动相为甲醇-水(17∶83),检测波长为275 nm,流速为1.0 ml/min。结果:岩白菜素进样量在0.050 55～1.365 00μg线性关系良好(r=0.999 9),平均回收率为99.5%,RSD为2.1%。结论:该方法简便、准确、重现性好,可用于该制剂中岩白菜素含量的测定。Objective： To establish a method for content determination of bergenin in Kaihoujian Spray. Methods： The HPLC method was performed on Hypersil ODS2-C18 （5 μm, 200 mm × 4. 6 mm） column with methanol-water （17：83 ） as mobile phase. Detection wavelength was 275 nm, and the flow rate was 1.0 ml/min. Results： The linear range of bergenin was 0. 050 55 - 1. 365 00 μg （ r = 0. 999 9）. The mean recovery was 99. 5%, and RSD was 2. 1%. Conclusions： The method is simple and accurate for determination of hergenin content in this drug.

关 键 词：开喉剑喷雾剂 岩白菜 色谱法 高压液相 

分 类 号：R927.2[医药卫生—药学]