microRNA-122在甲型肝炎患者血清中的表达  

The Expression of MicroRNA-122 in Serum of Patients with Hepatitis A

作　　者：朱海涛[1] 董琼珠[2] 贾户亮[2] 钦伦秀[2] 

机构地区：[1]贵阳医学院附院肝胆外科,贵州贵阳550004 [2]复旦大学附属中山医院,上海200032

出　　处：《贵阳医学院学报》2012年第3期270-272,共3页Journal of Guiyang Medical College

摘　　要：目的:探讨甲型肝炎病毒感染患者血清中miR-122表达水平与健康人及乙型肝炎病毒感染患者的差异。方法:24例甲型肝炎患者、22例乙型肝炎患者及25例健康人血清分别抽提总RNA,用实时定量PCR分析其中miR-122的表达并比较差异。结果:甲型肝炎患者血清中miR-122水平与健康人比较无显著差异(P>0.05),乙型肝炎患者血清中miR-122水平较甲型肝炎患者及健康人均显著升高(P<0.05)。结论:miR-122在甲型肝炎患者血清中较健康人无显著差异,而乙型肝炎患者血清中miR-122表达高于正常水平,提示miR-122可能不适合作为甲型肝炎相关肝病的标记物。Objective： To compare the serum level of miR-122 in hepatitis A virus-infected patients with both that of healthy people and hepatitis B virus-infected patients. Methods： Serum total RNA of 24 hepatitis A virus-infected patients, 22 hepatitis B virus-infected patients and 25 healthy people were extracted, and their expression levels of miR-122 were detected with quantitative real-time polymerase chain reaction analyses and compared. Results： No statistical difference was found in serum levels of miR-122 between hepatitis A virus-infected patients and healthy people （ P 〉 0. 05 ）. Serum level of miR-122 in hepatitis B virus-infected patients was higher than that of hepatitis A virus-infected patients and healthy people （P 〈0. 05）. Conclusions： The results indicate the serum miR-122 level may not be used as biomarker for hepatitis A virus-related hepatic disease.

关 键 词：微RNAS 肝炎 甲型 肝炎 乙型 聚合酶链反应 血清学 

分 类 号：R446.79[医药卫生—诊断学]