褪黑激素对大鼠睾丸扭转缺血和再灌注损伤的保护作用  

Protective effects of melatonin to testicular ischemia-reperfusion injury in rats

作　　者：徐波[1] 陈海琛 黄国显 徐延波 武东珍[1] 

机构地区：[1]哈尔滨医科大学附属第二医院小儿外科,150086 [2]厦门市妇幼保健院小儿外科

出　　处：《中华小儿外科杂志》2012年第7期532-535,共4页Chinese Journal of Pediatric Surgery

摘　　要：目的探讨褪黑激素对大鼠睾丸扭转的治疗作用。方法选取青春期雄性SD大鼠48只，随机分为3组：空白对照组（A组）；扭转复位组（B组）；扭转复位＋褪黑激素组（C组）。B组和C组大鼠建立睾丸扭转复位模型，对照组不扭转。扭转4h后复位睾丸，复位前15minB组腹腔注射生理盐水1ml；C组腹腔注射褪黑激素1ml（17mg／kg）。复位后4h处死所有动物取睾丸待测。以原位缺口末端标记法（TUNEL）检测生精细胞凋亡指数；化学比色法测定睾丸组织内总抗氧化能力（T-AOC）。结果B组T-AOC（20．31±2．55）U／mg比A组（33．62±3．29）U／mg明显降低，差异有统计学意义（P〈0．01）。而C组T-AOC（30．05±2．08）U／mg较B组明显升高（P〈0．05）。B组凋亡指数（42．2±3．21）％明显高于A组（5．7±0．67）％（P〈0．01），而C组凋亡指数（12．2±1．34）％较B组显著下降（P〈0．05）。结论褪黑激素具有明显对抗睾丸扭转复位后的氧化损伤，对因睾丸扭转导致的缺血再灌注损伤具有保护作用。Objective To examine the protective effects of melatonin to testicular ischemia- reperfusion injury caused by testicular torsion-torsion in rats. Methods Forty-eight adolescent male SD rats were randomly and evenly divided into 3 groups： A, control group; B, torsion-torsion group; C, torsiontorsion ＋ melatonin treatment group. The rats of group B and C underwent a spermatic cord torsion lasting 4 hours. The rats of the control group were subjected to sham operation. In the group C rats, 1 ml melatonin （17 mg/kg） was injected intraperitoneally 15 min before the cord was un- twisted. The group B rats were injected with lml saline 4 hours after detorsion, all animals were sacri- ficed, and their testis were harvested for germ cell apoptosis （TUNEL） and testicular tissue total an- tioxidant capacity （T-AOC） assay. Results T-AOC in the testis of group B rats decreased significantly compared with that of the A group rats （P〈0. 01 ）, and TAOC in the testis of group C rats increased significantly compare to that of the group B rats （P〈0. 05）. The apoptosis index in the testis of group B rats was significantly higher than that of group A rats （P〈0.01）, while the apoptotic index in the testis of group C rats decreased significantly compare to that of group B and C rats （P〈0.05）. Conclusions Melatonin prevents testis from oxidative damage after testicular ischemia-reperfusion injury caused by testicular torsion in rats.

关 键 词：褪黑激素 睾丸 再灌注损伤 

分 类 号：R726.9[医药卫生—儿科]