Megsin基因转染对小鼠肾小球系膜细胞单核细胞趋化蛋白-1及细胞间黏附分子-1表达的影响  

Effects of megsin gene transfection on the expressions of MCP-1 and ICAM-1 in mouse glomerular mesangial cells

作　　者：刘洁[1,2] 李英[1] 刘茂东[1] 任广伟[1] 王明铭[1] 李英敏[3] 丛斌[3] 

机构地区：[1]河北医科大学第三医院肾内科,河北石家庄050051 [2]河北大学附属医院内分泌科,河北保定071000 [3]河北医科大学法医学教研室,河北石家庄050051

出　　处：《细胞与分子免疫学杂志》2012年第7期702-705,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省自然科学基金资助项目(C2010000583)

摘　　要：目的:观察megsin基因转染对高糖环境中肾小球系膜细胞单核细胞趋化蛋白-1(MCP-1)及细胞间黏附分子-1(ICAM-1)表达的影响。方法:高糖环境中培养小鼠肾小球系膜细胞,分别培养12、24、48 h,采用MTT法检测细胞增殖程度,免疫细胞化学和Western blot法检测系膜细胞megsin、MCP-1、ICAM-1蛋白表达水平,ELISA检测细胞培养上清Ⅳ型胶原浓度。结果:高糖环境中肾小球系膜细胞megsin、MCP-1及ICAM-1表达增强,细胞增殖明显,细胞上清液中Ⅳ型胶原浓度升高,megsin基因转染后上述变化趋势更加显著,而megsin shRNA质粒转染可明显减弱上述变化。结论:Megsin可上调MCP-1及ICAM-1表达,促进系膜细胞增殖及系膜外基质积聚。AIM： To observe the effects of megsin gene transfection on the expressions of monocyte chemoattractant protein-1 （MCP-1） and intercellular adhesion molecule- 1 （ ICAM-1 ）. METHODS： Mouse glomerular mesangial cells were cultured in high glucose medium, and then cell prolifer- ation was measured by MTT assay at 12, 24 and 48 h respectively. The expressions of megsin, MCP-1 and ICAM-1 in mesangial cells were detected by immunocytochemical staining and Western blotting. The concentration of type Ⅳ collagen in the culture supernatant of mesangial cells was measured by ELISA. RESULTS： Under high glucose concentration, the expressions of megsin, MCP-1 and ICAM-1 increased; the concentration of type IV collagen in the cell supernatant was elevated as well; and mesangial cell proliferation was enhanced. After megsin gene transfection, the above changes were more significant, but were abated following megsin shRNA/transfection. CONCLUSION： Megsin gene can up-regulate the expressions of MCP-1 and ICAM- 1, promote mesangial cell proliferation and mesangial extracellular matrix accumulation.

关 键 词：MEGSIN 系膜细胞 基因转染 单核细胞趋化蛋白-1 细胞间黏附分子-1 

分 类 号：R392.12[医药卫生—免疫学]