大肠癌患者与正常健康人淋巴细胞表型差异分析  

Differential analysis of lymphocyte subsets between colorectal carcinoma patients and healthy individuals

作　　者：张国庆[1] 焦顺昌[1] 温新宇[2] 赵宏[1] 林星石[3] 吴亮亮[3] 

机构地区：[1]解放军总医院肿瘤内科,北京100853 [2]解放军总医院生化科,北京100853 [3]解放军总医院肿瘤中心实验室,北京100853

出　　处：《细胞与分子免疫学杂志》2012年第7期735-737,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家科技部支撑计划基金(2009BAI86B05)

摘　　要：目的:分析大肠癌患者与正常健康人20项淋巴细胞亚群变化情况。方法:流式细胞术(FCM)检测168例大肠癌患者与102例正常健康人外周血20项淋巴细胞亚群,SPSS17.0软件进行统计学处理。结果:与正常健康人相比,大肠癌患者CD29+、45RA+、CD4+CD45RO+和CD8+CD28-细胞百分比例明显升高,而NKT、45RO+、CD4+CD45RA+、CD28+和CD8+CD28+细胞百分比例则明显降低(P<0.05)。Ⅳ期患者组CD8+CD28-细胞百分比例明显高于正常健康人组、Ⅰ+Ⅱ期患者组及Ⅲ期患者组(P<0.05)。结论:大肠癌患者的细胞免疫功能降低,体液免疫功能有所增强;传统指标难以准确评价患者的免疫功能,包括CD8+CD28+、CD8+CD28-在内的多项细胞亚群分析体系也许能更好的反映大肠癌患者的免疫状况。AIM： To investigate the differences of twenty lymphocyte subsets between colorectal carcinoma patients and healthy individuals. METHODS： Twenty peripheral blood lymphocyte subsets from 168 colorectal carcinoma patients and 102 healthy individuals were examined by flow cytometry （FCM）. The differences of these lymphocyte subsets between healthy individuals and colorectal carcinoma patients at different clinical stages were analyzed statistically with SPSS 17.0 software. RESULTS： Compared with healthy people, the percentages of CD29^＋, 45RA^＋, CD4^＋ CD45RO＋ and CD8^＋ CD28^- cells in peripheral blood of colorectal carcinoma patients increased significantly （ P 〈 0.05）, while the percentages of NKT, 45RO^＋, CD4^＋ CEH5RA^＋ , CD28^＋ and CD8^＋ CD28^＋ cells decreased signifi- cantly （P 〈 0.05 ）. The percentage of CD8^＋ CD28^- cells in Ⅳ stage patients was significantly higher than that in healthy individuals, I ＋ II and III stage patients （ P 〈 0. 05）. CONCLUSION： The cellular immunity of colorectal carcinoma patients is attenuated, while the humoral immunity is strengthened. The traditional lymphocyte analysis using CD3^＋, CD4^＋, NK or CD4^＋/CD8^＋ ratio can not precisely evaluate the immunological function of colorectal carcinoma patients. The analysis of multi-lymphocyte subsets including CD8^＋ CD28^＋ and CD8 ＋ CD28 - cells, is likely a better way to evaluate the immunological status of colorectal carcinoma patients.

关 键 词：大肠癌 淋巴细胞亚群 临床分期 流式细胞仪 

分 类 号：R392.3[医药卫生—免疫学]