丝裂原活化蛋白激酶抑制剂对脓毒症大鼠肺损伤的保护作用  

Roles of Mitogen-Activated Protein Kinase in Pulmonary Inflammatory Response in Septic Rats

作　　者：王松柏[1] 姜龙 齐绍鸿[1] 张河[1] 

机构地区：[1]中国人民解放军第251医院麻醉科,河北张家口075000 [2]河北省涿鹿县县医院麻醉科,河北涿鹿075600

出　　处：《河北北方学院学报（自然科学版）》2012年第3期62-65,共4页Journal of Hebei North University:Natural Science Edition

摘　　要：目的探讨丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)活化与脓毒症大鼠肺组织和血浆肿瘤坏死因子-α(TNF-α)表达及组织炎症反应的关系。方法采用盲肠结扎穿孔术(CLP)模型制造大鼠腹腔感染。随机将56只大鼠分为正常对照组、CLP组和CLP+MAPK抑制剂(SB203580)处理组,各组又分不同时间点亚组。采用反转录多聚酶链式反应和酶联免疫吸附试验检测试剂盒分别测定血浆和肺TNF-αmRNA和蛋白水平;同时测定肺髓过氧化酶(MPO)活性、HE染色病理切片检测肺组织炎症反应程度。结果与正常大鼠相比,CLP后各时间点肺血浆和肺组织TNF-αmRNA、蛋白含量均升高明显;同时肺MPO和组织病理学评分升高明显。MAPK抑制剂处理后,与CLP组相应时间点相比,MPO和病理学评分均显著下降;TNF-αmRNA表达和蛋白含量同时显著降低。结论抑制MAPK活化可缓和脓毒症所致大鼠肺组织炎症反应,减轻肺组织损伤。Objective To investigate the effect of mitogen-activated protein kinase （MAPK） on ex- pression of tumor necrosis factor-α （TNF-α） and pulmonary injury induced by cecal ligation puncture （CLP） in septic rats. Methods Sepsis of rats was induced by CLP. 56 male SD rats were randomly di- vided into normal control, CLP group, and inhibitor （SB203580） of mitogen-activated protein kinase （MAPK） pre-treatment group. At serial time points in each group, animals were sacrificed. Then, pul- monary tissue and serum samples were harvested to determine TNF-α mRNA expression levels by reverse transcription polymerase chain reaction and its protein expression levels by enzyme-linked immunosorbent assay. Meanwhile, activity of myeloperoxidase and histopathology were also evaluated. Results Com- pared with that of normal control, pulmonary myeloperoxidase, histopathologic scoring as well as TNF-α mRNA and protein expression levels in lung or serum markedly increased respectively in CLP group. Fol- lowing pre-treatment with SB203580, MPO and histopathologic scoring reduced compared with that of CLP group, except for TNF-α mRNA and protein levels. Conclusion These data suggest that severe in- tra-abdoment infection could induce injury of remote lung and inhibiting the activation of MAPK could at- tenuate pulmonary tissue damage.

关 键 词：丝裂原活化蛋白激酶 肿瘤坏死因子-Α 大鼠 休克 

分 类 号：R631[医药卫生—外科学]