硒对大鼠肝细胞和储脂细胞过氧化脂质及细胞外基质的影响  

Effects of selenium on production of basic lipid peroxidation and excretion of extracellular matrix in rat cultured hepatocytes and Ito cells

作　　者：高润平[1] 王淑华[1] 李广生[1] 

机构地区：[1]白求恩医科大学,吉林长春130021

出　　处：《中国地方病学杂志》2000年第3期180-182,共3页Chinese Jouranl of Endemiology

基　　金：吉林省科技发展基金 !(2 0 0 0 30 0

摘　　要：目的　观察微量元素硒 (Se)对大鼠肝细胞、储脂 (Ito)细胞过氧化脂质和细胞外基质 (ECM)产生的影响。方法　采用原位灌注方法分离大鼠肝细胞和 Ito细胞。结果　培养肝细胞、Ito细胞有一定水平的基础丙二醛(MDA)产生和 ECM分泌。Se可提高肝细胞、Ito细胞谷胱甘肽过氧化物酶 (GSH- Px)活性 ,抑制基础 MDA产生 ,减少 3H-脯氨酸 (3H- Pro)掺入、 型前胶原 (PC )分泌及 Ito细胞透明质酸 (HA)分泌。结论　 Se可抑制肝细胞和 Ito细胞过氧化脂质产生和Objective Study the inhibitory effects of selenium on the production of basic lipid peroxidation and the secretion of extracellular matrix(ECM) of hepatocytes and ito cells in vitro.Methods Rat hepatocytes and ito cells were isolated and cultured.Results The results showed that selenium could obviously lower the production of malondialdehyde(MDA) and raise the activity of glutathione peroxidase(GSH Px) and inhibit the secretion of precollagen type Ⅲ (PCⅢ) in hepatocytes and ito cells as well as inhibit the secretion of hyaluronic acid in ito cells,and that there was a negative correlation between the level of PCⅢ and the ratio of GSH Px/MDA in both cells and a negative correlation between the levels of hyaluronic acid and the ratio of GSH Px/MDA in ito cells.Conclusions These studies indicated that selenium could lower the production of lipid peroxidation and inhibit the secretion of ECMs by raising the activity of GSH Px of rat hepatocytes and ito cells. [

关 键 词：硒 肝细胞 储脂细胞 脂质过氧化 细胞外基质 

分 类 号：R599[医药卫生—内科学]