光纤生物传感器检测嗜肺军团菌方法的建立  

Establishment of method of fiber optic biosensor for detection of Legionella Pneumophila

作　　者：宋玲玲[1] 陈苏红[2] 张敏丽[2] 张宏刚[2] 肖瑞[2] 王升启[2] 

机构地区：[1]军医进修学院,北京100853 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国医药导报》2012年第18期23-25,共3页China Medical Herald

基　　金：国家自然科学基金(31100712)

摘　　要：目的建立光纤生物传感器检测嗜肺军团菌的方法,为嗜肺军团菌提供一种快速、现场的检测手段。方法先用抗原或抗体包被光纤,再加入待测物质孵育6-10 min,最后加入荧光探针反应6-10 min,根据理化换能元件可以将荧光信号转换成电信号的原理,实现对样品的定性或定量检测目的。同时探讨了光纤修饰方法,包被缓冲液以及离子强度等因素对实验结果的影响。结果光纤生物传感器法检测嗜肺军团菌时,最低可检测到3×104cfu/mL,检测响应时间为20 min;伤寒沙门菌、大肠杆菌、结核杆菌、小肠结肠炎耶尔森菌、霍乱弧菌、金黄色葡萄球菌、痢疾志贺菌的检测结果均为阴性,显示出良好的特异性。结论光纤生物传感器检测嗜肺军团菌时,表现出快速、灵敏度高、特异性强、重复性好等优点,可作为嗜肺军团菌的一种初筛方法。Objective To establish the method of fiber optic biosensor for the detection of Legionella Pneumophila, in order to offer a fast, field detection mean for Legionella pneumophila. Methods At first, antigen or antibody was used to coat fibers; then the sample was added to incubate about 6 to 10 minutes; finally, the fluorescent probe was added to react 6 to 10 minutes. The purpose of qualitative or quantitative determination for the sample could be achieved, depending on the electrical signals converted from fluorescent signals by physieochemieal transducer. Meanwhile, the effects of fiber modification methods, coated buffers and ionic strength on experimental results had been studied. Results When fiber optic biosensor used for Legionefla pneumophila detection, its limit detection can achieve as low as 3 -10^4 CFU/mL and its response time was about 20 min. The detection results of Salmonella Typhi, Escherichia Coli, Mycobacterium Tuberculosis, Yersinia Enterocolitica, Vibrio Cholerae, Staphylococcus Aureus and Shigella Dysenteriae were all negative, which showed favourable specificity. Conclusion Fiber optic biosensor shows advantages of rapid, high sensitivity, good specificity and reproducibility in detection of Legionella pneumophila. It can be used as a screening method for Legionella pneumophila detection.

关 键 词：嗜肺军团菌 光纤生物传感器 光纤修饰 

分 类 号：R345[医药卫生—基础医学]