Th1/Th2细胞在慢性乙型肝炎病毒感染中的作用  

Role of T helper cells in chronic hepatitis B virus infection

作　　者：姜荣龙[1] 卢桥生[1] 郭亚兵[1] 侯金林[1] 冯筱榕[1] 章廉[1] 骆抗先[1] 富宁[2] 

机构地区：[1]第一军医大学南方医院感染内科,广东广州510515 [2]第一军医大学免疫学教研室,广东广州510515

出　　处：《第一军医大学学报》2000年第2期103-105,共3页Journal of First Military Medical University

摘　　要：目的了解慢性ＨＢＶ感染者外周血单核细胞（ＰＤＭＣ）中ＣＤ４~＋Ｔ细胞内ＩＬ－４和ＩＦＮ－γ的表达情况，以测定Ｔｈ０／Ｔｈ１／Ｔｈ２细胞的百分数，探明Ｔｈ１、Ｔｈ２细胞在慢性ＨＢＶ感染中的作用。方法常规分离ＰＢＭＣ，在ＰＭＡ、ｉｏｎｏｍｙｃｉｎ、莫能菌素的刺激下，采用流式细胞检测（ＦＡＣＳ）对慢性ＨＢＶ感染者ＰＢＭＣ中ＣＤ４~＋Ｔ细胞内ＩＬ－４和ＩＦＮ－γ表达进行分析。结果正常对照组，２．３％－１８.６％的ＣＤ４~＋Ｔ细胞为Ｔｈ１细胞，０.９％－９．２％为Ｔｈ２细胞；在慢性ＨＢＶ感染者外周血单个ＣＤ４~＋Ｔ细胞中，以Ｔｈ０细胞居多：而Ｔｈ１细胞则随着慢性乙型肝炎肝脏炎症活动的加剧而明显增多，在炎症活动明显的慢性乙型肝炎患者中，Ｔｈ１细胞百分数明显高于炎症活动呈静止状态的慢性乙型肝炎；Ｔｈ２细胞在慢性ＨＢＶ感染的不同阶段则较为恒定，但明显高于对照组。结论Ｔｈ１细胞与炎症活动呈正相关，Ｔｈ２细胞可能与ＨＢＶ感染的慢性化相关联。Objective The ratio of Th0/Th1 /Th2 cells in chronic HBV-infected individuals was investigated by determining the expression of IL-4/IFN-γ in Single CD4^+ T cells isolated from peripheral blood. and the role of Th1 /Th2 cytokines in chronic HBV-infection was analyzed. Methods Peripheral blood mononuclear cells (PBMCs) from chronically HBV-infected individuals were isolated routinely, stimulated by PMA, ionomycin and monensin, and the production of IL-4/IFN-γby CD4^+ T cells in PBMC was determined by FACS analysis. Results The percentage of IFN-γ-producing T cells and IL-4 producing T cells ranged from 2.3% to 18.6% and from 0.9% to 9.2% respectively in CD4^+ cells in non-infected individuals The majority of CD4^+ T cells from PBMCs in chronic HBV-infected individuals were Th0 cells. The percentage of Th1 cells increased significantly with the hepatic inflammation activity The percentage of Th1 cells was higher in advanced stage than in stationary phase of chronic hepatitis B, The percentage of Th2 cells in CD4^+ T cells from HBV-infected individuals showed no significant difference at different stages, but was higher than that from controls. Conclusion The results indicated that Th1 phenotype cytokines were associated with hepatic inflammation activity of chronic hepatitis B and Th2 cells may be associated with the chronicity of HBV Infection.

关 键 词：慢性乙型肝炎 TH1/TH2细胞 IL-4 IFN-Γ 

分 类 号：R512.6[医药卫生—内科学]