监测FasL表达评估异基因造血干细胞移植后T细胞免疫功能状态  

Investigation of the FasL expression to evaluate the immunoactivity of T lymphocytes in patients undergoing allogeneic hemopoietic stem cells transplantation

作　　者：冯晓勤[1] 周淑芸[1] 冯茹[1] 伍伯松 杨艺[1] 张建军[2] 王随照[3] 朱为国[4] 

机构地区：[1]第一军医大学南方医院血液科,广东广州510515 [2]广东省人民医院流式细胞室,广东广州510080 [3]广东省人民医院流式血液科,广东广州510080 [4]第一军医大学南方医院儿科,广东广州510515

出　　处：《第一军医大学学报》2000年第2期109-112,共4页Journal of First Military Medical University

基　　金：广东省自然科学基金!(980222)

摘　　要：目的评估异基因造血干细胞移植后Ｔ淋巴细胞免疫功能状态。方法采用三色荧光标记的流式细胞术，对４例异基因造血干细胞移植、２例自体干细胞移植患者移植前后的Ｔ淋巴细胞及其亚群的ＦａｓＬ表达进行监测。结果移植前Ｔ细胞的ＴａｓＬ、表达率仅（２.１３％±２.０２％），与正常对照组无显著差异（Ｐ＞０.０５），移植后异基因造血干细胞移植患者ＦａｓＬ表达水平升高，尤以急性ＧＶＨＤ患者表达升高显著（Ｐ<０.０５），而自体干细胞移植患者的ＦａｓＬ表达未见明显改变。结论异基因造血干细胞移植术后部分Ｔ淋巴细胞处于激活状态； ＦａｓＬ表达可能与ａＧＶＨＤ相关；对ＦａｓＬ表达的监测可从细胞、免疫分子两个层次对Ｔ细胞免疫功能状态进行评估，该体系的完善对临床判断预后、指导治疗可能有较直接的参考价值。objective To evaluate the immunoactivity of T lymphocytes in patients after allogeneic hemopoietic stem cells transplantation (allo-HSCT) Methods Four patients undergoing allo-HSCT and 2 patients undergoing autogenous hemopoietic stem cells transplantation (auto-HSCT) were investigated for the expression of FasL among lymhocyte subsets before and after transplantation using three-color flow cytometry. Results The expression of FasL.in T cells was very low before transplantation (2.13±2.02%), which showed no significant difference from that in normal controls (P >0.05) The expression of FasL. increased after allo-HSCT, which was most obviously demonstrated in 2 patients with acute graft versus host disease (aGVHD) (P <0.05). Significant increase of FasL. in T cells was not observed in patients undergoing auto-HSCT, Conclusions A proportion of T lymphocytes were activated after allo-HSCT and the expression of FasL may be related with aGVHD The expression of FasL in T cells serves as a parameter in the cytological and immunomolecular evaluation of the immunoactivity of T cells. This method may be of value in judging the clinical prognosis and provides experimental information for clinical treatment

关 键 词：异基因造血干细胞移植 细胞免疫 FASL T细胞 

分 类 号：R392.4[医药卫生—免疫学]