几种阿魏酸衍生物高分子药物对血小板聚集和血栓素B_2、6-酮-前列腺素F_(Ⅰα)产生的影响  

Influences of ferulate acid, indomethacin, indomethacinum-ferulate acid and imdomethacinum-ferulate acid-starch on rabbit platelet aggregation and production of thromboxan B_2 and 6-keto-prostaglandin F_(Ⅰα) in vitro

作　　者：杨俊旺[1] 梁永录 肖丽英 李天锡[2] 单春文[3] 林继红 

机构地区：[1]解放军兰州医学高等专科学校药理学教研室,甘肃兰州730020 [2]兰州医学院药化教研室,甘肃兰州730000 [3]第一军医大学药理教研室,广东广州510515 [4]第一军医大学实验动物教研室,广东广州510515

出　　处：《第一军医大学学报》2000年第2期152-154,共3页Journal of First Military Medical University

摘　　要：目的通过测定兔血小板聚集和血栓素Ｂ２（ＴＸＢ２）、６－酮－前列腺素Ｆ_(Ⅰα)（６－ｋｅｔｏ－ＰＧＦ_(Ⅰα)）的含量，比较观察阿魏酸（Ｆ）、吲哚美辛（Ｉ）、阿魏酸－吲哚美辛结合物（ＦＩ）和阿魏酸－吲哚美辛－淀粉高分子载体衍生物（ＦＩＳ）在抗血栓方面的药理作用特点。方法用比浊法和放免法测定Ｆ、Ｉ、ＦＩ和ＦＩＳ在体外对兔血小板聚集和对ＴＸＢ２，６－ｋｅｔｏ－ＰＧＦ_(１α)生成的影响。结果Ｆ、Ｉ、ＦＩ和ＦＩＳ显著地抑制ＡＤＰ和ＡＡ诱导的兔血小板聚集和ＴＸＢ_２，６－ｋｅｔｏ－ＰＧＦ_(Ⅰα)的产生。在 １２．５－２４０μｍｏｌ／Ｌ的剂量范围内，对血小板聚集的抑制率， Ｆ为 １１．３％－３１．８％（ＡＤＰ诱导）和 ３.３％－１８．５％（ＡＡ诱导）、Ｉ为３％－３４．３％（ＡＤＰ诱导）和２％－２０．２％（ＡＡ诱导）、ＦＩ为５．０％－５９．３％（ＡＤＰ诱导）和０．３％－６３．１％（ＡＡ诱导）、ＦＩＳ为３.１％－３９．１％（ＡＤＰ诱导）和１．５％－３９.６％（ＡＡ诱导）。剂量为 ２４０.０μｍｏｌ／Ｌ时对ＴＸＢ_２和６－ｋｅｔｏ－ＰＧＦ_(Ⅰα)产生的抑制率， Ｆ为 １４．８％和 １.２％、Ⅰ为 ２４.９％和 ２６．２％、 ＦＩ为 ４０.６％和２９．３％?objective To investigate the effect of indomethacinum-ferulate acid (FI) and indomethacinum-ferulate acid-starch (FIS) on rabbit platelet aggregation induced by ADP and AA and on the production of thromboxane B2 (TXB2) and 6-keto-prostaglandin F_(Ⅰα)(6-keto-PGF_(Ⅰα)) Methods Turbimetry and radioimmunoassay were employed to measure in vitro the platelet aggregation and the content of TXB_2, 6-keto-PGF_(Ⅰα) production in rabbit blood treated with ferulate acid (F), indomethacin (I), FI and FIS respectively. Results It was shown that F, I, FI and FIS significantly inhibited ADP-induced rabbit platelet aggregation and the production of TXB_2 and 6-keto-PGF_(Ⅰα) With the dosage of 12.5 to 240.0 μmol/L, the inhibition rate (%) of platelet aggregation by F was 11.3%-31.8% (ADP-induced) and 3.3%-18.5%(AA-induced), that by I was 5.3%-34.3% (ADP-induced) and 6.2%-20.2% (AA-induced), that by FI was 25.0%-59.3% (ADP-induced) and 30.3%-63.1% (AA-induced), and that by FIS was 13.0%-39.1% (ADP-induced) and 11.5%-39.6%(AA-induced) With the dosage of 240.0 μmol/L, the inhibition rates of TXB_2 and 6-keto-PGF_(Ⅰα) production by F was 14.8% and 1.2%.that by I was 24.9% and 26.4%. that by FI was 40.6% and 29.3% and that by FIS was 28.7% and 21.4%. The ratio of 6-keto-PGF_(Ⅰα) to TXB_2 wes 2.0 (control group), 2,4 (F), 2.0 (I), 2.4 (FI) and 2.2 (FIS),

关 键 词：TXB2 血小板聚集 6-KETO-PGF1Α 阿魏酸衍生物 

分 类 号：R96[医药卫生—药理学]