普罗布考对过氧化氢诱导平滑肌细胞增殖的影响  

Effects-of Probucol on smooth muscle cell proliferation induced by H_2O_2

作　　者：顾江涛[1] 吴宗贵[1] 沈茜[2] 徐玉莲[2] 

机构地区：[1]第二军医大学长征医院心血管内科,上海200003 [2]长海医院实验诊断科

出　　处：《第二军医大学学报》2000年第4期362-365,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目!(39670316)

摘　　要：目的：探讨普罗布考（Ｐｒｏｂｕｃｏｌ）对Ｈ２Ｏ２诱导的平滑肌细胞（ＳＭＣＳ）增殖的抑制作用。方法：采用细胞计数法和＾３Ｈ－ＴｄＲ掺入法观察０．１～１００μｍｏｌ／Ｌ Ｐｒｏｂｕｃｏｌ对新生牛血清（ＮＣＳ）和过氧化氢（Ｈ２Ｏ２）促ＳＭＣＳ增殖的抑制作用。结果：１０μｍｏｌ／Ｌ及１００μｍｏｌ／Ｌ Ｐｒｏｂｕｃｏｌ作用９６ｈ，可抑制１０％ＮＣＳ刺激的ＳＭＣＳ增殖，而１，１０及１００μｍｏｏｌ／ＬObjective: To investigate the inhibitory effects of Probucol on smooth muscle cells (SMCs) proliferation. Methods: SMCs treated with H_2O_2 and new born calf serum(NCS) served as proliferative cellular model in vitro. Inhibition effects of Probucol on the SMCs proliferation was detected by cell counting and incorporation of ~3H-TdR. Results: A does- dependent inhibition effects on the proliferation of SMCs induced by 10% NCS was observed when treated with 10 μmol/L and 100 μmol/L Probucol for 96 h (P<O. 05, P<0. 01 ). Does-dependent inhibition effects on proliferation of SMCs evoked by H_2O2_ was found when treated with 1 μmol/L, 10 μmol/L and 100 μmol/L Probucol for 72, 96 h (P<0. 05, P<O. 01 ). Con- clusion: Probucol inhibits SMCs proliferation stimulated by NCS or H_2O_2 Probucol may have potential clinical implication in the prevention and treatment of atherosclerosis and restenosis after percutaneous transluminal coronary angioplasty.

关 键 词：普罗布考 平滑肌细胞 动脉粥样硬化 细胞增殖 

分 类 号：R543.505[医药卫生—心血管疾病] R972.6[医药卫生—内科学]