p16甲基化与宫颈疾病的相关性研究  

Association between p16 gene methylation and cervical diseases

作　　者：姚红霞[1] 王玲[1] 吴红[1] 陈琦[1] 

机构地区：[1]同济大学附属第一妇婴保健院妇科,上海200050

出　　处：《同济大学学报（医学版）》2012年第3期17-19,36,共4页Journal of Tongji University(Medical Science)

摘　　要：目的探讨p16基因甲基化与宫颈疾病的相关性。方法分别采用甲基化特异性PCR(MethylationspecificPCR,MSP)法和免疫组化法检测宫颈上皮内瘤样病变组织和宫颈癌组织中p16基因甲基化发生率和P16蛋白表达的缺失率。结果正常宫颈中未检测到p16基因甲基化,低度宫颈癌前病变p16基因甲基化率为3.33%。中度及高度宫颈癌前病变p16基因甲基化率分别为20.0%及26.7%。宫颈癌p16基因甲基化率为44.0%。中、高度病变及宫颈癌组与正常宫颈及低度病变甲基化率相比P<0.05,宫颈癌与中、高度病变组甲基化率相比P<0.05。结论 p16基因甲基化与宫颈疾病的发展具有相关性。Objective To investigate the association between p16 gene methylation and cervical diseases. Methods Immunohistocbemistry and methylation-specific PCR （MSP）were performed on 90 samples of cervical intraepithelial（CIN）and 25 samples of cervical carcinoma. Results The expressions of p16 gene methylation in normal cervical tissue and CINI were lower（0.0% and 3.33% ）than that in CINII（ 20. 0% ）, CINII1（26.7% ）and cervical carcinoma（44.0% ）. Conclusion There is correlation of p16 gene methylation with the development of cervical diseases.

关 键 词：宫颈疾病 P16基因 甲基化 

分 类 号：R713.4[医药卫生—妇产科学]