HPLC对肺结核患者利福平血药浓度的监测  

Monitoring of plasma rifampicin concentrations in patients with pulmonary tuberculosis by HPLC

作　　者：张利斌[1] 张晓庆[1] 郝晓晖[2] 李玉平[1] 

机构地区：[1]同济大学附属肺科医院药剂科,上海200433 [2]同济大学附属肺科医院结核科,上海200433

出　　处：《同济大学学报（医学版）》2012年第3期64-67,共4页Journal of Tongji University(Medical Science)

基　　金：上海市结核重点实验室开放基金项目(2009K04)

摘　　要：目的建立肺结核患者给予利福平后血药浓度的监测方法,为临床合理用药提供参考。方法高效液相法,色谱柱为安捷伦C18柱,(5μm,4.6×250mm);流动相为甲醇:乙腈:0.04mol×L^(-1)KH_2PO_3(30:30:40);流速1.2ml/min,检测波长为340nm;柱温20℃。结果血浆中利福平在0.30～24μg/ml范围内浓度与峰面积线性关系良好,r=0.9995,平均回收率为98.87%。140例肺结核患者静滴或口服利福平450mg后,其中126例血药浓度检测结果在0.50～12.75μg/ml之间,12例血药浓度检测结果0.30～0.5μg/ml之间,2例血药浓度小于0.3μg/ml。结论该方法准确、灵敏,专属性强,重现性好,适用于治疗药物监测,可为临床合理用药提供数据参考。Objective To monitor plasma rifampicin concentrations in patients with pulmonary tuberculosis by HPLC. Methods The assay was conducted on C18 GL sciences column（5μm, 4.6 × 250 mm） with the mobile phase of the mixture of CH3OH-CH3CN-0.04 mol/L KH2PO3 （30： 30： 40）at a rate of 1.2 ml/min, the UV detection wavelength was 340 nm. The column temperature was 20 ℃. Results A good linearity was obtained from 0. 30 -24 μg/ml with a correlation coefficient of 0. 999 5. The average recovery of the assay was 98.87%. Among 140 patients administrated with 450 mg rifampicin orally or intravenously, the plasma drug concentration ranged 0. 50 - 12.75 μg/ml in 126 cases, ranged 0. 30 -0.5 μg/ml in 12 cases and was less than 0.3μg/ml in 2 cases. Conclusion The method applied in this study is accurate and sensitive, which can be used to monitor plasma rifampicin concentration for rational drug administration in clinical practice.

关 键 词：利福平 高效液相色谱法 血药浓度 

分 类 号：R521[医药卫生—内科学]