3T3-L1脂肪细胞胰岛素抵抗模型建立的3种方法对葡萄糖转运时效关系的影响  

Time-Effect Study of Glucose Transportation in Insulin Resistance Models of 3T3-L1 Adipocytes by Three Different Methods

作　　者：陈立[1] 杨明炜[2] 库宝庆[1] 马聪[1] 莫阔[1] 汪继敏[1] 

机构地区：[1]湖北省襄阳市中心医院,湖北襄阳441021 [2]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《武汉大学学报（医学版）》2012年第4期491-494,共4页Medical Journal of Wuhan University

基　　金：湖北省卫生厅2004年度中医药中西结合课题(编号:2006AA301B16)

摘　　要：目的:对比3种不同方法(地塞米松、游离脂肪酸、高糖高胰岛素)诱导的胰岛素抵抗3T3-L1脂肪细胞的葡萄糖转运时效关系及对葡萄糖转运子4(Glut4)蛋白表达的影响。方法:采用3种不同方法诱导3T3-L1脂肪细胞产生胰岛素抵抗,分别检测各组细胞12,24,36,48及60h时的葡萄糖转运率及各组细胞60h后Glut4蛋白的表达。结果:在24h内,造模组细胞的葡萄糖转运率与正常对照组相比均明显降低(P<0.01);在24h以后,游离脂肪酸组葡萄糖转运率与正常对照组相比无明显统计学差异(P>0.05),地塞米松组葡萄糖转运率相比正常对照组明显降低(P<0.05),而高糖高胰岛素组葡萄糖转运率较地塞米松组明显降低(P<0.01);地塞米松组及游离脂肪酸组的Glut4蛋白表达水平较正常对照组明显降低(P<0.01);相对于地塞米松组及游离脂肪酸组,高糖高胰岛素组的Glut4蛋白表达水平明显降低(P<0.01)。结论:高糖高胰岛素能够诱导3T3-L1脂肪细胞产生更强的胰岛素抵抗,这种抗性的产生可能是因为抑制了Glut4蛋白的表达获得的。Objective： To observe the glucose transportation and Glut4 expression in the induction of insulin resistance in 3T3-L1 adipocytes by three methods（dexamethasone,free fatty acids,and high glucose/hyperinsulinism）.Methods： Insulin resistance was induced in three different model groups of 3T3-L1 adipocytes according to the three different methods.The glucose transportation rate was detected by the uptake of 2-deoxy——D-glucose in each group after 12,24,36,48 and 60 hours.The Glut4 expression was assayed by means of Western blot after 60 hours.Results： Within 24 hours,as compared with normal control group,the glucose transportation in each model group decreased significantly（P0.01）.After 24 hours,as compared with that in normal control group,the glucose transportation rate in free fatty acid group showed no difference（P0.05）,but decreased obviously in dexamethasone group（P0.05）,while the rate in high glucose and hyperinsulinism group decreased significantly even than that in dexamethasone group（P0.01）.The Glut4 expression in dexamethasone group and free fatty acids group both decreased significantly as compared with normal control group（P0.01）.And compared with that respectively in dexamethasone group and free fatty acids group,the Glut4 expression in high glucose and hyperinsulinism group decreased significantly（P0.01）.Conclusion： High glucose and hyperinsulinism can induce 3T3-L1 adipocytes to produce stronger insulin resistance,and the production of the resistance may be due to the inhibition of Glut4 expression.

关 键 词：3T3-L1脂肪细胞 胰岛素抵抗 细胞模型 葡萄糖转运 葡萄糖转运子4 

分 类 号：R598.2[医药卫生—内科学]