腺苷脱氨酶、结核抗体及DNA联合检测在结核性积液的临床诊断价值  

Clinical Diagnosis Value of Adenosine Deaminase,Mycobacterium Tuberculosis Antibody and DNA Combined Detection in Tuberculous Effusion

作　　者：何丙生 代秀萍 李双初 张晓霞 王俊 

机构地区：[1]湖北省孝感市结核病防治所,湖北孝感432000

出　　处：《武汉大学学报（医学版）》2012年第4期544-546,共3页Medical Journal of Wuhan University

摘　　要：目的:探讨腺苷脱氨酶(ADA)、结核抗体(TB-Ab)、TB-DNA联合检测在结核性积液的临床诊断价值。方法:选择87例胸腔积液进行分析,其中结核组38例,非结核组49例,比较3种检测方法的检测情况及单独检测和联合检测的方法学指标。结果:两组别3种检测方法的阳性率经卡方检验分析,有统计学差异(P<0.05),在结核组中3种检测方法阳性率经卡方检验分析比较,有统计学差异(P<0.05),3种检测方法以TB-DNA阳性率最高,3种方法联合检测灵敏度为92.1%,特异度以TB-DNA最高为97.9%。结论:3种方法联合检测可提高灵敏度,提高结核杆菌的检出率,方法学指标综合分析,以TB-DNA检测为优。Objective： To explore the diagnosis value of combined detection of adenosine deaminase（ADA）,mycobacterium tuberculosis antibody and DNA（TB-Ab and TB-DNA） in tuberculous effusion.Methods： Eighty-seven samples of body cavity effusions from tuberculosis patients（n=38） and non tuberculosis patients（n=49） were abstracted for ADA,TB-Ab and TB-DNA detections.Results： There were significant differences between the two groups in all the three indices by chi-square test（P0.05）,and among the three indices in the positive rate（P0.05）.Positive rate of TB-DNA was the highest,specificity of TB-DNA was the highest as 97.9%,and the sensitivity of combined detection of the three indices sensitivity was increased to 92.1%.Conclusion： Three indices combined detection can improve the sensitivity and detection rate of mycobacterium tuberculosis,and TB-DNA testing is the best in the three indices.

关 键 词：腺苷脱氨酶 结核抗体 结核杆菌 结核性积液 

分 类 号：R521.7[医药卫生—内科学]