骨髓间充质干细胞分泌因子对Ⅰ型变态反应的影响  

THE EFFECT OF CYTOKINE SECRETED BY BONE MARROW MESENCHYMAL STEM CELLS ON TYPEⅠALLERGIC REACTION

作　　者：高明敏[1] 刘晓萍[2] 于业军[1] 王家辉[1] 贾跃伟 李薇[2] 

机构地区：[1]青岛大学医学院附属医院皮肤科,山东青岛266003 [2]青岛大学医学院组织胚胎学教研室 [3]奥克生物开发有限公司

出　　处：《青岛大学医学院学报》2012年第3期218-220,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科技局资助课题(07-2-1-4-nsh)

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)分泌因子对Ⅰ型变态反应的影响。方法采用全骨髓贴壁法分离和纯化大鼠BMSCs,收集培养第3代的BMSCs上清液获取干细胞分泌因子。制备大鼠抗卵蛋白血清;分离培养大鼠腹腔肥大细胞,用大鼠抗卵蛋白血清特异性致敏肥大细胞,甲苯胺蓝染色法观察干细胞分泌因子对肥大细胞脱颗粒的影响;制备大鼠同种被动皮肤过敏动物模型(PCA),观察干细胞分泌因子对其影响。结果干细胞分泌因子和酮替芬对特异性致敏肥大细胞脱颗粒和大鼠PCA均有明显抑制作用,与模型组相比差异有显著性(F=219.79～357.32,q=21.26～34.05,P<0.01)。结论干细胞分泌的细胞因子对Ⅰ型变态反应有抑制作用。Objective To explore the effect of cytokine secreted by bone marrow mesenchymal stem cells （BMSCs） on type Ⅰ allergic reaction. Methods Rat BMSCs were isolated and purified by adherence screening. The stem cell cytokine was obtained from the supernatants of third passage BMSCs. Rat ovaserum was prepared, and mast cells were isolated from rat peritoneal cavity and cultured. The mast cells were then allergized by the ovaserum, the influence of excreted factors of stem cells on degranulation of mast cells was observed by toluidine blue staining. A passive cutaneous anaphylaxls （PCA） model in rats was created to observe the effect of BMSCs on the model. Results Both BMSCs and ketotifen showed a significant inhibition on the degranula tion of mast cells and PCA model （F=219. 79--357. 32 ;q=21. 26-34. 05 ;P〈0.01）. Conclusion BMSCs cytokine secreted by BMSCs may inhibit type Ⅰ allergic reaction.

关 键 词：骨髓 间质干细胞 干细胞因子 超敏反应 大鼠 WISTAR 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]