四氯化碳中毒大鼠肝脏Ca^(2+)-ATP酶和磷酸化酶a活性的研究  

Studies on the Activities of Ca^(2+)-ATPase and Phosphorylase a in the Liver of CCl_4-poisoned Rats

作　　者：赵宝昌[1] 姚晓丹[1] 顾淑珍[1] 杜国忠[1] 欧阳启楣 林钧材[1] 

机构地区：[1]大连医学院生化教研室

出　　处：《中华劳动卫生职业病杂志》1990年第4期215-217,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：本实验采用雄性S.D.大鼠,经日一次灌注2.2ml/kg 四氯化碳,于染毒后12、24、48小时测定肝微粒体Ca^2+-ATP 酶和肝细胞液磷酸化酶a 活性变化。结果发现,染毒后大鼠肝微粒体Ca^2+-ATP 酶活性显著降低,以染毒后24小时为甚;肝细胞液磷酸化酶a 活性显著增高,以染毒后24小时为最高。说明四氯化碳致肝损伤时,肝内质网隔离钙的能力降低,胞液中钙浓度升高。本实验对于阐明四氯化碳中毒性肝病发病机理有一定的意义。Carbon tetrachloride was administered to male Sprague-Dawley rats in asingle hepatotoxic dose of 2.2ml/kg.The activity of liver-microsomalCa^(2+)-ATPase and liver-cytosol phosphorylase a was measured at 13,24 and48hr postadministration.The results were that the activity of Ca^(2+)-ATPasewas remsrkably decreased,while of phosphorylase a was highly increased.These data demonstrated that the ability of hepatic endoplasmic reticulum tosequester calcium is destroyed during CCl_4 poisoning.causing calcium to ac-cumulate in liver-cytosol.This experiment has certain significance in revealingthe mechanism of hepatosis induced by CCl_4 poisoning.

关 键 词：四氯化碳中毒 肝脏 磷酸化酶 

分 类 号：R595[医药卫生—内科学]