氧化苦参碱对H_2O_2诱导的L_6成肌细胞凋亡的影响  

Effect of Oxymatrine on H_2O_2 Resistance in L_6 Rat Myoblast Cells

作　　者：朱道立[1] 王康乐[1] 陈佩林[1] 姜雅琼[1] 

机构地区：[1]南通大学生命科学学院,南通226019

出　　处：《生物化学与生物物理进展》2012年第7期662-670,共9页Progress In Biochemistry and Biophysics

基　　金：南通大学博士点建设专项(05024276);南通市科技局社会发展科技计划资助项目(S2008022)~~

摘　　要：研究氧化苦参碱对L6大鼠成肌细胞H2O2凋亡的影响.采用过氧化氢损伤L6大鼠成肌细胞的方法,建立L6大鼠成肌细胞H2O2凋亡模型.使用剂量为0.3,0.15,0.75 g/L的氧化苦参碱处理细胞.应用MTT法统计存活率和流式细胞仪检测细胞周期及凋亡率,用DAPI荧光染色、HE染色以及Bax和Bcl-2抗体鉴定损伤程度,Western blot检测蛋白质差异.结果表明,H2O2损伤的成肌细胞存活率降低,凋亡率增加.各种剂量氧化苦参碱能提高成肌细胞的存活率,促使Bcl-2增高,Bax降低.对成肌细胞的保护程度随氧化苦参碱剂量增加而增强,在剂量为0.3 g/L时,效果显著,其次是0.15、0.75 g/L的氧化苦参碱.其生理生化机制是氧化苦参碱保护H2O2通过NFκB信号通路造成的大鼠成肌细胞凋亡模型.结果显示,氧化苦参碱具有作为新的抗氧化药物的潜力.Effect of oxymatrine on the H2O2 of L6 rat myoblast cells was investigated in this study. The hydrogen peroxide（H2O2） was used to establish the H2O2-induced apoptosis model by L6 rat myoblast cells that were treated with 0.3, 0.15, 0.75 g/L oxymatrine. The survival rate was measured by MTT method, cell cycles and apoptosis rate of L6 rat myoblast cells were detected by flow cytometry, the injury degree was determined by apoptosis cytochemistry fluorescent antibody of Bcl-2 and Bax, DAPI staining and HE staining. The protein differences were examined by Western blot. Results showed that survival rate of L6 myoblast cells was degraded and the apoptosis rate was increased by damaging H2O2. The survival rate of L6 myoblast cells was heightened by oxymatrine of each dose. They could induce the increase in expression of Bcl-2 and the decrease in expression of Bax. Its degree of protection was increased with raising the dosage of oxymatrine and the protective effect of 0.3 g/L dosage is obvious. The protective effect of 0.15, 0.75 g/L oxymatrine were secondary. The main physiological and biochemical effects of oxymatrine was related to protect rat myoblast apoptosis model through NFKB signal transduction. These results implied that oxymatrine may have the potential as a new antioxidant treatment medicine.

关 键 词：氧化苦参碱 H2O2诱导凋亡模型 L6大鼠成肌细胞 BCL-2 BAX 

分 类 号：Q593.3[生物学—生物化学] Q26