日本血吸虫尾蚴感染小鼠CD8α^-CD11c^+型树突状细胞对哮喘的抑制作用  

The Inhibitory Effect of CD8 α^-CD11c^+ Dendritic Cells on Asthma in Schistosoma Japonicum Infected Mice

作　　者：路平[1] 朱云娟[1] 刘俊燕[1] 吴增强[1] 安桂珍[1] 纪伟华[1] 任继玲[1] 刘佩梅[1] 

机构地区：[1]天津医科大学基础医学院寄生虫学教研室,300070

出　　处：《天津医药》2012年第7期695-697,I0003,共4页Tianjin Medical Journal

基　　金：天津市自然科学基金项目(项目编号:11JCZDJC19500)

摘　　要：目的:研究日本血吸虫尾蚴感染鼠CD8α-CD11c+型树突状细胞(DC)在抑制过敏性哮喘中的作用。方法:用磁珠分选方法纯化日本血吸虫尾蚴感染鼠及正常鼠DC亚群CD8α-CD11c+细胞。将20只BALB/c小鼠随机均分为4组,A组为过继转移日本血吸虫尾蚴感染鼠CD8α-CD11c+型DC并诱发过敏性哮喘组,B组为过继转移正常鼠CD8α-CD11c+型DC并诱发过敏性哮喘组,C组为单纯过敏性哮喘组,D组为生理盐水对照组。A、B组小鼠经尾静脉过继转移5×105个DC,1h后A、B、C3组均开始诱发哮喘。4周后处死小鼠,取小鼠肺组织进行病理学检查。结果:肺部病理学结果显示A组小鼠肺组织炎症明显减轻,支气管管壁基本光滑,支气管内无黏液分泌。B组、C组肺组织炎症反应严重,支气管管壁增厚,管内存在大量黏液。D组小鼠肺组织无炎症反应。病理评分结果A组高于D组,低于B、C组,差异有统计学意义(P<0.05或P<0.01),B组和C组的差异无统计学意义(P>0.05)。结论:日本血吸虫尾蚴感染鼠CD8α-CD11c+型DC可明显抑制过敏性哮喘的发生。Objective： To study the inhibitory effect of CD8α^- CD11c^＋.dendritic ceils （DC） on allergic asthma in schistosoma japonicum-infected mice. Methods： CD8α^- CD11c^＋ DC from schistosoma japonicum-infected mice and naive were isolated and purified using microbeads respectively. Twenty BALB/c mice were divided into four groups randomly. CD8α^- CD11c^＋ DCs （5 × 10^5） from schistosoma japonicum-infected mice were injected into the tail vein of mice in group A.CD8α^- CD11c^＋ DCs （5×10^5） of naive were injected into the tail vein of mice in group B. Group C included simple allergic asthma rats. Rats in group D were given normal saline. One hour after injection, mice were sensitized and challengedwith ovalbumin to induce asthma in groups A, B and C. After four weeks, all mice were killed, and left lungs were collected for histopathological analysis. Results： Pathology results showed that pulmonary inflammation diminished significantly in mice of group A. There were more severe lung infection in groups B and C. No lung infection was found in group D. Results of lung pathological score was significantly higher in group A than that of group D, but significantly lower than that of groups B and C （P〈 0.05 or P〈 0.01）. There was no significant difference between group B and group C （P〉 0.05）. Conclusion： CD8α^- CD11c^＋ DC can significantly inhibit allergic asthma in schistosoma japonicum infected mice.

关 键 词：血吸虫病 日本 哮喘 树突细胞 过继转移 小鼠 近交BALB C疾病模型 动物 

分 类 号：R532.21[医药卫生—内科学]