高通量基因芯片初步筛选ITP脾和正常脾巨噬细胞的差异表达基因  

Association of Differentially Expressed Genes in the Macrophages of Spleen in Patients with Idiopathic Thrombocytopenic Purpura by cDNA Microarray

作　　者：王艺超[1,2] 彭兵[1] 吴仲[1] 陈思瑞[1] 李永彬[1] 王昕[1] 

机构地区：[1]四川大学华西医院肝胆胰外科,成都610041 [2]成都军区总医院全军普通外科中心,成都610083

出　　处：《四川大学学报（医学版）》2012年第4期525-529,535,共6页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅科技计划项目(No.2008SZ0195)资助

摘　　要：目的研究免疫性血小板减少性紫癜(ITP)患者脾和正常脾巨噬细胞的差异表达基因。方法提取9例ITP患者及9例正常人脾巨噬细胞的总RNA,反转录制备含荧光分子Cy5标记的cDNA探针,与含有30968点cDNA的表达谱芯片杂交后扫描荧光强度,筛选出差异表达的基因并进行分析。结果基因芯片共筛选出1545个差异表达基因,其中上调基因718个,下调基因827个。差异表达基因涉及免疫反应、细胞黏附及受体调节、细胞信号转导、细胞骨架和运动代谢、细胞凋亡、酶调活性等方面。差异表达基因生物路径涉及Toll样受体信号通路,Fcγ受体介导吞噬通路,促分裂原活化蛋白激酶信号通路、细胞内吞通路等。结论基因芯片筛选ITP患者脾巨噬细胞的差异表达基因是有效的。差异表达基因可能为ITP发病机制的研究提供新证据和治疗靶标。Objective To study the differentially expressed genes of splenic macrophage in patients with immune thrombocytopenic purpura.Methods Macrophages were isolated from the spleen.Total RNA of the macrophages were extracted and reversely transcript into cDNA.cDNAs were labeled with Cy5,then hybridized with cDNA chips containing 30968 genes.The gene chips were scanned and analyzed for the differentially expressed genes.Results A total of 1545 differentially expressed genes were identified by cDNA chip.718 genes were highly expressed and 827 genes were down-regulated.The differently expressed genes include those involved in immunologic response,cell adhesion,cell signal transduction,cytoskeleton,exercise metabolism,apoptosis,enzyme regulator activity and so on.The pathway association analysis were related with Toll-like receptor pathway,Fc gamma mediated phagocytosis,MAPK signaling pathway,endocytosis.Conclusion cDNA mircroarray is an effective technique in screening for differentially expressed genes of the macrophages in patients with ITP.Further analysis of the obtained genes will help understanding the pathogenesis of ITP,and the therapeutic targets.

关 键 词：免疫性血小板减少性紫癜 基因芯片 脾脏 巨噬细胞 

分 类 号：R346[医药卫生—基础医学]