黄连温胆汤加减对代谢综合征大鼠肿瘤坏死因子-α和白介素-6的影响  

Effect of Huanglianwendan Decoction on Tumornecrosis Factor-α and Interleukin-6 in Metabolic Syndrome Rats

作　　者：刘莉[1] 许锋锐[1] 芦晔[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《湖北中医药大学学报》2012年第3期11-13,共3页Journal of Hubei University of Chinese Medicine

基　　金：黑龙江省自然科学基金项目(D2007-85)

摘　　要：目的观察黄连温胆汤加减对代谢综合征(MS)大鼠血清肿瘤坏死因子(TNF-α)和白介素-6(IL-6)的影响。方法采用高盐高脂高糖饲料喂养,诱导实验性MS大鼠模型。大鼠随机分为模型组、黄连温胆汤加减高剂量组、黄连温胆汤加减低剂量组、盐酸二甲双胍对照组和空白组。生物化学法检测血糖、血清胰岛素的水平,放免法检测血浆TNF-α、IL-6水平。结果模型组血浆血清TNF-α、IL-6和胰岛素抵抗指数水平显著增加(P<0.01);黄连温胆汤加减高剂量组血清TNF-α、IL-6和胰岛素抵抗指数水平降低(P<0.05)。结论黄连温胆汤加减对MS大鼠胰岛素抵抗指数具有降低作用,其机制可能与降低TNF-α、IL-6水平有关。Objective To observe the effect of HUANGLIANWENDAN decoction on expression of interleukin -6 and tumor necrosis factor - ctin order to explore the function of the tre - atment mechanism of Insulin resistance. Methods Sixty male SD rats were randomly divided into normal diet group （n = 10）and high fat high salt and high glucose diet gr- oup （n = 50） to induce metabolic syndrome model then metabolic syndrome rats were got 37 successfully, and then these 37 metabolic syndrome rats were randomly divi - ded into model group （n = 9）, high dose HUANGLIANWENDAN decoction group （n = 10）, low dose HUANGLIANWENDAN decoction group （n = 10） and DM- BG group （n = 8 ）, normal diet group and model group were feed physiologic - al saline, fasting blood glucose （ FBG）, fasting insulin （FINS）, interleukin -6 （IL- 6） and tumor necrosis factor- α（TNF -α）in the serum were detected by enzymatic method, radioimmunoas - say, or enzyme - linked immunosorbent assay after 4weeks. Results In model group level of TNF - ct, IL - 6in blood and IR index are higher than normal diet group （P 〈0. 01 ）; The data in high dose HUANGLIANWENDAN decoction is depressed evidently （P 〈 0. 05）. Conclusion HUANGLIANWENDAN decoction can regulate IR index, and the mechanism may be that HUANGLIANWENDAN decoction can up - regulate the level of TNF - α.IL - 6 in blood.

关 键 词：代谢综合征 黄连温胆汤 胰岛素抵抗 肿瘤坏死因子 白介素-6 

分 类 号：R285.5[医药卫生—中药学]