HPLC法测定氨咖黄敏胶囊中马来酸氯苯那敏的含量  

Determination of Chlorphenamine Maleate in Ankahuangmin Capsule by HPLC

作　　者：郑阿利 李哲 

机构地区：[1]甘肃省庆阳市食品药品检验所,庆阳745000

出　　处：《中国药事》2012年第6期626-628,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立测定氨咖黄敏胶囊中马来酸氯苯那敏含量的方法。方法色谱柱为VP-ODS柱,流动相为甲醇-0.05moL·L-1磷酸二氢钾溶液(含三乙胺0.03%,用磷酸调pH至3.0)(43∶57),检测波长为215nm,柱温为40℃,流速为1.0mL·min-1。结果马来酸氯苯那敏在20.24～48.57μg·mL-1浓度范围内线性关系良好,r=0.9998;平均回收率为99.78%,RSD=0.67%。结论该方法专属性好,简捷、快速、准确,适用于氨咖黄敏胶囊中马来酸氯苯那敏含量的测定。Objective To establish an HPLC method for determination of Chlorphenamine Maleate in Ankahuangmin Capsules. Methods A Shim-pack VP-ODS column was used and the temperature was set at 40℃. The mobile phase consisted of methanol-0.05 mL·min-1 potassium dihydrogen phosphate solution （43 ： 57）, containing 0.03% triethylamine, and the PH value was adjusted to 3.0 with phosphate. The detection wavelength was set at 215 nm. Results There was a good linear in the range of 20.2-48. 57 mL·min-1（r=0. 9998）. The average recovery was 99. 78%, and RSD was 0. 67%. Conclusion A specific, simple, rapid, accurate and suitable method is established for determination of Chlorphenamine Maleate in Ankahuangmin Capsules.

关 键 词：高效液相色谱法 氨咖黄敏胶囊 马来酸氯苯那敏 

分 类 号：R917[医药卫生—药物分析学] R927.2[医药卫生—药学]