2,9-二甲基-1,10-菲咯啉的dl-丙氨酸衍生物的合成表征及其镧(Ⅲ)配合物与 DNA作用光谱研究  

Synthesis, Characterization of dl-Alanine Derivative of 2, 9-Dimethyl-1, 10-Phenanthroline and DNA-binding Spectral Study of its Lanthanum (Ⅲ) Complex

作　　者：汪中明[1] 周志芬[1] 林华宽[1] 朱守荣[1] 刘天府[1] 陈荣悌[1] 

机构地区：[1]南开大学化学系,天津300071

出　　处：《无机化学学报》2000年第3期503-509,共7页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金!(No. 29671020);天津市自然科学基金

摘　　要：以２，９－二甲基－１，１０－菲咯啉为初始原料，合成了２，９－二甲基－１，１０－菲咯啉的ｄｌ－丙氨酸衍生物：１，１０－菲咯啉－２，９－二亚甲基亚氨基－（２，２’－二甲基）二乙酸（Ｌ）。该配体经过元素分析。红外光谱、核磁共振氢谱表征。在 ２５±０．１℃、 Ｉ＝ ０．１ｍｏｌ·ｄｍ^（－３）ＮａＮＯ_３的条件下，用ｐＨ电位滴定法测定了该配体的质子化常数及其与Ｌａ（Ⅲ）的配合物的稳定常数。通过电子光谱研究了Ｌｂ（Ⅲ）的配合物与小牛胸腺 ＤＮＡ的相互作用。用溴化乙锭作为荧光探针研究了 Ｌａ（Ⅲ）－Ｌ配合物与小牛胸腺 ＤＮＡ的相互作用的过程。作为对比，也研究了 Ｌａ（Ⅲ）分别与１，１０－菲咯啉（Ｐｈｅｎ）、 ｄｌ－丙氨酸（ Ａｌａ）的配合物与小牛胸腺 ＤＮＡ的相互作用。结果表明 Ｌａ（Ⅲ）－Ｌ配合物与小牛胸腺ＤＮＡ作用既有共价键合，又有插入作用。Starting from 2, 9-dimethyl-1, 10-phenanthroline, its dl-alanine derivative: 1, 10-phenanthroline -2, 9-dimethanoimino- (2, 2'-dimethyl) diacetic acid (L) was synthesized and characterized by elemental analysis, IR, ~1HNMR. The protonation constants of the ligand L, the stability constants of La (Ⅲ) -L binary complexes have been determined by pH titration method at 25 ±0. 1℃, I= 0. 1mol. dm-3 NaNO3. The interaction of the lanthanum (Ⅲ) complex with calf-thymus DNA was studied by electronic spectra. Using ethidium bromide as a fluorescence probe, binding style of the lanthanum (Ⅲ complex with calf-thymus DNA was studied by spectroscopy. For comparison, the interaction of the complexes of lanthanum (Ⅲ) with 1, 10-phenanthroline and dl-alanine with calf-thymus DNA were also studied, respectively. The results indicate that the lanthanum (Ⅲ) complex possibly interacts with calf-thymus DNA by both intercalative and covalent binding.

关 键 词：1 10-菲咯啉 dl-丙氨酸衍生物 镧配合物 DNA 

分 类 号：O626[理学—有机化学] O614.331[理学—化学]