ATDC蛋白在大肠癌中的表达及意义  

Expression and distribution of ATDC in colorectal carcinomas

作　　者：邓军[1] 吴珂[1] 李航[1] 童强[1] 卢晓明[1] 王国斌[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胃肠外科Ⅱ、微创外科,武汉430022

出　　处：《腹部外科》2012年第3期179-181,共3页Journal of Abdominal Surgery

摘　　要：目的探讨共济失调性毛细血管扩张D组互补基因（ATDC）表达在大肠癌的发生发展中的作用及其临床意义。方法应用免疫组织化学方法检测大肠癌病人癌组织和正常大肠组织中的ATDC蛋白表达和定位，Westernblot和RT-PCR定量ATDC蛋白的表达水平，分析ATIX；蛋白和临床病理参数之间的关系。结果ATDC蛋白在正常组织和癌组织表达差异有统计学意义（P〈0．05），癌组织中ATDC表达在性别、年龄、Dukes分期因素之间差异无统计学意义，在淋巴结转移及分化程度因素间差异有统计学意义（P〈0．05）。结论ATDC在癌组织中表达升高提示其可能参与了肿瘤的发生发展。检测ATDC表达对于大肠癌的诊断和判断预后有一定的意义。Objective To investigate the impact of ataxia telangiectasia group D complement gene （ATDC） in the development of coloreetal cancer and the clinical significance. Methods The specimens from the patients with colorectal carcinomas were divided into colorectal cancer group and normal colorectal group （control group）. Immunohistochemistry was used to detect the expression and localization of ATDC protein. Western blot and quantitative RT-PCR were used to detect mRNA and protein expression of ATDC. The relationship between ATDC expression and clinicopathological pa- rameters was analyzed by using PASW Statistics18. Results There was significant difference in the ATDC protein expression between normal tissue and cancer tissue （P〈0. 05）. The ATDC expression has no relationship with gender, age and .dukes stage except lymph node metastasis and the degree of differentiation Conclusion The ATDC expression in colorectal carcinoma is increased, suggesting it may be involved in the process of tumor development. Detection and suppression of ATDC expression have great significance for the diagnosis and treatment of tumors. There is a correlation between AT- DC and the clinicopathological parameters of colorectal cancer, suggesting tha（ATDC at high levels of expression may have some clinical values for assessment of colorectal cancer treatment and prognosis.

关 键 词：结直肠肿瘤 基因调控网络 免疫组织化学 印迹法 蛋白质 逆转录聚合酶链反应 共济失调性毛细血管扩张D组互补基因 

分 类 号：R818[医药卫生—放射医学]