植物内生菌醇提取物对大豆凝集素的作用  

Effects of Endophytes Alcohol Extraction on Soybean Agglutinin Contents

作　　者：陈珣[1] 杨镇[1] 肖军[1] 龚娜[1] 王娜[1] 王红[1] 肇莹[1] 杨涛[1] 

机构地区：[1]辽宁省农业科学院微生物工程中心,辽宁沈阳110161

出　　处：《大豆科学》2012年第3期501-503,共3页Soybean Science

基　　金：辽宁省科技攻关项目(2011215005)

摘　　要：豆科植物凝集素在豆科植物和根瘤菌的相互识别和共生关系的建立上起着重要作用。利用血凝法快速检测R(人参)、S(沙棘)、D(越橘)3种植物内生菌的S0.25D50、S0.25、R25D25和R50D50共4种处理对大豆根部凝集素含量的影响。结果表明:经过S0.25D50、S0.25、R50D503种处理的大豆根部凝集素含量均高于对照,而经过S0.25处理的大豆根部凝集素的含量最高,为0.288 mg.g-1。说明沙棘内生菌醇提取物有利于大豆根瘤菌的形成。Legume agglutinins play important roles in mutual recognition and establishment of the symbiotic relationship between legumes and rhizobium.In this study,soybean agglutinin contents under four treatments（S0.25D50,S0.25,R25D25 and R50D50）were rapid detected using hemagglutination method.R,S and D were three endophytes from ginseng,sea-buckthorn and cowberry,respectively.The results showed that agglutinin contents in soybean roots under S0.25D50,S0.25 and R50D50 treatments were higher than the control.The highest root agglutinin content was 0.288 mg·g-1 under S0.25 treatment which indicated that alcohol extract of endophyte from sea-buckthorn contributed to the formation of soybean Rhizobium.

关 键 词：大豆 凝集素含量 植物内生菌 血凝法 

分 类 号：S565.1[农业科学—作物学]