2011年上半年四川省麻疹病毒分离株N基因序列分析  

Sequence Analysis on N Gene of Mealses Virus Isolated in Sichuan Province,2011

作　　者：刘李[1] 孙莉[1] 何吉兰[1] 杨勇[2] 童文彬[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]巴中市疾病预防控制中心

出　　处：《预防医学情报杂志》2012年第6期426-429,共4页Journal of Preventive Medicine Information

摘　　要：目的探讨四川省目前流行的麻疹病毒的基因型别和特征。方法采用Vero/SLAM细胞分离培养麻疹病毒,通过逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)方法扩增麻疹病毒N基因羧基末端456 bp片段,并对PCR产物进行序列测定和同源性分析。结果 2011年四川省分离到10株麻疹病毒均为H1a基因亚型,与H1基因型代表毒株的同源性为97.1%～98.9%。结论 H1a基因亚型仍为四川省本土流行病毒株的优势亚型,近年来四川省流行的麻疹病毒无较大变异,优势病毒的持续传播与麻疹的发病有密切联系。Objective To explore the genetic characteristics and genotype of measles virus recently prevalented in Sichuan.Methods Vero/ Slam cell were used for measles viral isolation and culture.A 456 bp fragment of the N gene was amplified by Reverse Transcription-Polymerase Chain Reaction（RT-PCR） and subjected to sequence and homological analysis.Results Ten strains of measles virus were isolated in 2011 in Sichuan province and were all identified as H1a subtype,N genes homology was 97.1%-98.9%.Conclusion H1a subtype was the predominant genotype in Sichuan province.In recent years,there was no large variation of H1a subtype circulated in Sichuan.The continuous spread of predominant strain is colsely related to the incidence of measles.

关 键 词：麻疹病毒 N基因 基因型 序列分析 

分 类 号：R373.11[医药卫生—病原生物学]