水稻橙红色突变体的遗传分析与基因定位  

GENETIC ANALYSIS AND MAPPING OF A GENE CONFERRING ORANGE-RED ON LEAVE AND HULL IN RICE

作　　者：蒲志刚[1,2] 张志勇[2] 张志雄[2] 向跃武[2] 蔡平钟[2] 张婷[2] 李仕贵[1] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]四川省农业科学院生物技术核技术研究所,四川成都610066

出　　处：《核农学报》2012年第3期416-419,438,共5页Journal of Nuclear Agricultural Sciences

基　　金：四川省财政基因工程(2011JYGC01-001)

摘　　要：从水稻材料H9808航天诱变的后代中筛选出1株与色素相关的突变体,该突变植株在幼穗分化期新出幼叶的叶脉为橙红色,到拔节后期植株节间及下部约1/3的茎秆均呈现橙红色,在茎杆节底部中心有一橙红色的原点,抽穗时颖壳为橙红色,并保持到种子成熟,遗传分析表明突变体受1对隐性基因控制。在突变体与9311杂交的F2代隐性群体中,利用SSR标记将橙红色基因定位在2号染色体RM5350和RM12601标记之间,遗传距离分别为0.55cM和1.38cM。再利用新设计的InDel标记,进一步将该基因定位在S1和S2之间,遗传距离分别为0.41和0.25,为克隆水稻橙红色基因奠定了基础。A pigment-related mutant which induced by space environment was discovered in the population of rice H9808,The mutant was characterized by young leaves with orange-red veins at the stage of panicle initiation.The internode and one third of the down part stem became orange-red by the end of booting stage.The color of the mutant hull is also orange-red from heading to maturity.Genetic analysis showed that the mutant was controlled by a single recessive gene.The gene located between SSR marker RM5350 and RM12601,and the genetic distance were 0.55cM and 1.38cM using the F2 genetic mapping population generated by crossing the mutant with 9311.Inorder to further locate the gene locus,2 polymorphic InDel markers were designed.Finally,the gene locus was mapped between InDel marker S1 and S2,the genetic distances were 0.41and 0.25.These results built a substantial foundation for cloning the rice orange-red gene.

关 键 词：水稻航天诱变 橙红色 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]