高能混合粒子场辐照对花生胚小叶组织培养及植株再生的影响  

EFFECTS OF MIXED HIGH ENERGY PARTICLE FIELD ON EMBRYONIC LEAFLET CULTURE AND PLANT REGENERATION OF PEANUT

作　　者：于新玲[1] 刘录祥[2] 乔利仙[1] 隋炯明[1] 郭宝太[1] 徐丽娟[1] 王晶珊[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/国家农作物航天诱变技术改良中心,北京100081

出　　处：《核农学报》2012年第3期433-438,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然基金项目(30871544);863课题(2012AA100402);山东省中青年科学家奖励基金(BS2009NY028)

摘　　要：采用不同剂量的高能混合粒子场(0、67、105、150和196Gy)辐照花生鲁花11号干种子,取其胚小叶外植体,先后置于添加10mg/L 2,4-D的MSB5诱导培养基和添加4mg/L BAP的MSB5培养基上进行培养,研究了不同剂量辐照对外植体存活率、体胚诱导率和植株再生率的影响。结果表明:外植体存活率,除67Gy辐照组和对照无显著差异外,其他处理随辐照剂量的增大而显著下降;体胚诱导率,辐照剂量为67Gy时和对照无显著差异,当辐照剂量分别增加至105Gy和150Gy时,其体胚诱导率显著低于67Gy时的体胚诱导率,而二者之间无显著差异,当辐照剂量再增加至196Gy时,未观察到体胚的形成;植株再生率,除105Gy和150Gy剂量时无显著差异外,其他处理随辐照剂量的增大而显著下降。在辐照剂量为105Gy时,外植体存活率、体胚诱导率和植株再生率均约为对照的一半,因此推断105Gy可作为鲁花11号适宜的诱变剂量。再生苗嫁接后移栽于试验田正常结实。In order to study effects of mixed high energy particle field on embryonic leaflet culture and plant regeneration of peanut（Arachis hypogaea L.）,dry seeds of peanut cultivar Luhua 11 were irradiated with five doses（0,67,105,150 and 196Gy）,then the embryonic leaflets were separated and incubated on MSB5 induction media containing 10mg/L 2,4-D,and the subcultures were subsequently transferred to MSB5 media supplemented with 4mg/L BAP.The results showed that the survival rate of explants decreased significantly with the increase of irradiation dose,except for that treated by 67Gy.Somatic embryo induction rate also showed no significant difference between CK and 67Gy,while that of 105Gy and 150Gy were much lower than that of 67Gy.Nevertheless,there was no somatic embryo formation in 196Gy.The frequencies of plant regeneration reduced remarkably with the increase of irradiation doses,except for that treated by 105Gy and 150Gy.According to the rate of survival explants,somatic embryo formation and plant regeneration,105Gy was the optimum mutation dose.The regenerated plantlets were grafted and transplanted to the field,and they eventually set seeds.

关 键 词：花生 混合粒子场辐照 离体诱变 体胚诱导 植株再生 

分 类 号：S565.2[农业科学—作物学]