过继转输TSA诱导的CD4^+CD25^+调节性T细胞对不明原因复发性流产的治疗作用  

The treating function of TSA induced CD4^+CD25^+ regulatory T cells in unexplained recurrent spontaneous abortion

作　　者：齐彦[1] 赵富玺[2] 郭俊成[2] 穆雅琴[2] 王键[2] 殷治华[2] 冯婷婷[1] 

机构地区：[1]山西医科大学基础医学院,太原030001 [2]山西大同大学医学院免疫研究所,大同037009

出　　处：《中国优生与遗传杂志》2012年第5期21-23,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金资助项目(81170621);国家计生委项目(C-73);省攻关项目(200803110722;0080311058)

摘　　要：目的观察过继转输TSA诱导的CD4+CD25+调节性T细胞(CD4+CD25+Treg)对不明原因流产的作用机制及妊娠预后的影响。方法以雌性CBA/J×雄性BALB/c为正常妊娠模型,以雌性CBA/J×雄性DBA/2J为自然流产模型,使用免疫磁珠方法分选雌性CBA/J小鼠脾脏CD4+CD25+Treg细胞,并使用流式细胞术检测分选纯度。采用TSA对流产孕鼠外周CD4+/CD25-T细胞Foxp3基因特定位点进行表观修饰,以实现Foxp3稳定、持久的表达,并将CD4+Treg分别转输至流产模型孕4d(着床期)的雌性CBA/J孕鼠,于孕14d分别观察宿主孕鼠的胚胎吸收率。结果与对照组比较,过继转输TSA诱导的CD4+CD25+Treg细胞的宿主孕鼠的胚胎吸收率(11.27%)显著下降。结论孕早期过继转输TSA诱导的CD4+CD25+Treg细胞疗法能诱导宿主母胎免疫耐受,有利于妊娠的维持。Objective： To investigate the mechanism and the effect of the adoptivetransfer of CIM ~ CD25 ~ regulatory T cells （ CD4^＋CD25^＋ Treg） from the TSA induced mice on pregnant outcome of the abortion - prone mice. Methods ： The experiments were performed in following two groups ： using female CBA/J × male BALB/c matings as the normal pregnancy model and female CBA/J x male DBA/2J matings as the abortion -prone model. CD4^＋CD25^＋ Treg were isolated by MACS （magnetic cell sorting）, FCM （flow cytometry） is to analyse the purity of CD4^＋CD25^＋ Treg. In order to achieve the stable and lasting expression of Foxp3, TSA is applied to the apparent heredity beautification of the special location of Gene Foxp3 in CD4^＋、CD25^＋ T for miscarried mice. Purified CD4^＋CD25^＋ Treg cells were obtained from spleens of the virgin CBA/J mice and pregnant CBA/J mice at day 14 of gestation respectively, and then injected intravenously into the pregnant CBA/J mice mated with DBA/2J males at day 4 of gestation （time of implantation）, then the embryo resorption rate was counted at day 14 of gestation. Results： Compare with control group, the embryo resorption rate （11.27% ） after adoptive transfer of TSA induced pregnancy in CD4^＋CD25^＋ Treg decreased significantly. Conclusion： The adoptive transfer of TSA - induced CD4^＋CD25^＋ regulatory T cells therapy can induce the maternal - fetal immuno - tolerance and maintaining pregnancy.

关 键 词：曲古菌素A CD4+CD25+调节性T细胞 过继转输 胚胎吸收率 

分 类 号：R714.21[医药卫生—妇产科学]