转SrPCS2烟草的获得及Cd抗性评价  

Study on Cadmium Resistance of Transgenic Tobacco Expressing Sesbania rostrata Phytochelatin Synthase2

作　　者：李安明[1] 李德华[1] 邓青云[1] 汪宜宇[1] 

机构地区：[1]孝感学院生命科学技术学院,孝感432000

出　　处：《吉林农业大学学报》2012年第3期260-263,275,共5页Journal of Jilin Agricultural University

基　　金：湖北省自然科学基金项目(2005ABA083)

摘　　要：由已克隆得到的长喙田菁(Sesbania rostrata)植物螯合肽合成酶SrPCS2编码177个氨基酸,以pHAN-NIBAL及pART27为基础,构建了CaMV35S启动子驱动的SrPCS2基因植物表达载体pAM23,采用电击转化方法将pAM23导入根癌农杆菌EHA105,并用该菌株对烟草进行了转化,Northern-blot分析结果表明得到了转录该基因的烟草,但转录该基因的烟草没有提高对Cd的抗性。The phytochelatin synthase （SrPCS2） cDNA full-length encoding 177 amino-acids was ob-tained from Sesbania rostrata. The CaMV35S promoter driving plant expression on vector pAM23 with Sr-PCS2 was constructed based on the pHANNIBAL and pART27. By electroporation transformation, pAM23 was transferred into Agrobacterium tumefacien EHA105 and the new engineering bacterium was transferred into Nicotiana tabacum. Transgenic tobacco expressed SrPCS2 was identified by Northern-blot, but the transgenic tobacco did not enhance cadmium resistance.

关 键 词：植物螯肽合成酶 植物表达载体 转化 抗性 

分 类 号：S572[农业科学—烟草工业]