宫内苯并(a)芘暴露对胎儿癌症相关基因表达的影响  

Impacts of Prenatal Benzo(a)pyrene Exposure on the Expression of Cancer-related Gene of Fetus

作　　者：李永红[1] 程义斌[1] 顾珩[1] 顾雯[1] 金银龙[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所

出　　处：《环境卫生学杂志》2012年第3期101-104,111,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：卫生行业科研专项项目(201002001);"十一.五"国家科技支撑计划项目(NO.2006BAI19B01);国家环境与疾病监测点项目;美国NIH Fogarty项目(5D43 TW007864-09)

摘　　要：目的初步探讨宫内苯并(a)芘(BaP)暴露对胎儿癌症相关基因表达的影响。方法用高效液相色谱法检测20份新生儿脐带血血清中BaP的浓度,并按照BaP浓度将样本分成不同暴露组,用实时荧光定量PCR检测脐带血RPL27A(ribosomal protein L27a)、NUDT2(Nudix(nucleoside diphosphate linked moiety X)-type motif 2)和E2F1(E2F transcription factor 1)基因的表达,分析不同BaP暴露水平与癌症相关基因表达的关系。结果 20份脐带血血清BaP平均浓度为0.38±0.31μg/L。按照血清BaP浓度,将20份样本分成5个不同水平的暴露组。随着BaP暴露水平的增高,RPL27A和NUDT2呈现表达上调的趋势,而E2F1呈现表达下调的趋势。结论宫内BaP暴露可引起胎儿癌症相关基因表达的改变。Objectives To explore the impacts of prenatal benzo （a）pyrene （BaP） exposure on the expression of cancer-related gene of fetus. Methods BaP in cord serum of 20 newborns was detected by using high-performance liquid chromatography （HPLC）/fluorescence, and the mRNA of RPL27A, NUDT2 and E2F1 in cord serum was tested by real-time PCR. The relationship between BaP exposure level and gene expression was analyzed. Results The average level of BaP in 20 cord serum samples was 0. 38 ±0. 31 ug/L and the samples were grouped into 5 groups according to BaP levels. With the increase of BaP exposure, the expression of RPL27A and NUDT2 genes showed an up-regulated trend, while E2F1 gene showed a down-regulated trend. Conclusion Prenatal BaP exposure might induce a change of the expression of cancerrelated genes.

关 键 词：苯并(A)芘 基因表达 宫内暴露 脐带血 REAL-TIMEPCR 

分 类 号：R714.2[医药卫生—妇产科学]