Characterization of Strain Pseudomonas sp. Q1 in Microbial Fuel Cell for Treatment of Quinoline-Contaminated Water  

作　　者：ZHANG Cui-Ping CHEN Shah-Shan LIU Guang-Li ZHANG Ren-Duo XIE Jian 

机构地区：[1]South China Sea Marine Engineering and Environment Institute,SOA,Guangzhou 510300(China) [2]School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510275(China) [3]Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology,Guangzhou 510275(China)

出　　处：《Pedosphere》2012年第4期528-535,共8页土壤圈（英文版）

基　　金：Supported by the National Natural Science Foundation of China (Nos. 51039007 and 51179212);the Special Fund of State Key Joint Laboratory of Environment Simulation and Pollution Control of China (No. 10K04ESPCT);the Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, China (No. 2011K0001);the Guangdong Provincial Program of Production, Teaching and Research (No. 2009B090300324);the Major Projects of Special National Science and Technology of Water (No. 2009ZX07528-001)

摘　　要：To find new strain in the microbial fuel cell (MFC) for quinoline removal from wastewater and soil, a facultative anaerobic bacterium strain was isolated from the anode of MFC, utilizing quinoline as the carbon source and electron donor. Based on the 16S rRNA sequence analysis, the bacterium strain was Gram-negative and identified as Pseudomonas sp. Q1 according to its morphology and physiochemical properties. The strain was inoculated into a double-chambered MFC using various quinoline concentrations (0, 50, 75, 86, 100, 150, 200 and 300 mg L-1 ) combining with 300 mg L-1 glucose as the fuel. Results showed that electricity was generated from the MFC, in which quinoline was degraded simultaneously. The values of Coulombic efficiency (CE) increased with the increase of quinoline concentrations from 0 to 100 mg L-1 then decreased with the increase of quinoline concentration from 100 to 300 mg L-1 , and the maximum CE 36.7% was obtained at the quinoline concentration of 100 mg L-1 . The cyclic voltammetry analysis suggested that the mechanism of electron transfer was through excreting mediators produced by the strain Q1. The MFC should be a potential method for the treatment of quinoline-contaminated water and soil.To find new strain in the microbial fuel cell （MFC） for quinoline removal from wastewater and soil, a facultative anaerobic bacterium strain was isolated from the anode of MFC, utilizing quinoline as the carbon source and electron donor. Based on the 16S rRNA sequence analysis, the bacterium strain was Gram-negative and identified as Pseudomonas sp. Q1 according to its morphology and physiochemical properties. The strain was inoculated into a double-chambered MFC using various quinoline concentrations （0, 50, 75, 86, 100, 150, 200 and 300 mg L-1） combining with 300 mg L^-1 glucose as the fuel. Results showed that electricity was generated from the MFC, in which quinoline was degraded simultaneously. The values of Coulombic efficiency （CE） increased with the increase of quinoline concentrations from 0 to 100 mg L^-1 then decreased with the increase of quinoline concentration from 100 to 300 mg L^- 1, and the maximum CE 36.7% was obtained at the quinoline concentration of 100 mg L^-1. The cyclic voltammetry analysis suggested that the mechanism of electron transfer was through excreting mediators produced by the strain Q1. The MFC should be a potential method for the treatment of quinoline-contaminated water and soil.

关 键 词：bacterium strain BIODEGRADATION electron transfer power generation GLUCOSE 

分 类 号：Q939.112[生物学—微生物学] TM911.45[电气工程—电力电子与电力传动]