Allelic Variation at the TaZds-A1 Locus on Wheat Chromosome 2A and Development of a Functional Marker in Common Wheat  

作　　者：DONG Chang-hai MA Zhi-ying XIA Xian-chun ZHANG Li-ping HE Zhong-hu 

机构地区：[1]College of Agronomy, Agricultural University ofHebei, Baoding 071001, P.R.China [2]Institute of Crop Sciences, National Wheat Improvement Center~The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China [3]CIMMYT China Office/CAAS, Beijing 100081, P.R.China [4]Beijing Engineering and Technique Research Center of Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, P.R. China

出　　处：《Journal of Integrative Agriculture》2012年第7期1067-1074,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (30871522 and 30830072);the National Basic Research Program of China(2009CB118300);an Earmarked Fund for the Modern Agro-Industry Technology Research System, China (CARS-3-1-3)

摘　　要：ζ （zeta）-carotene desaturase （ZDS） is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment （YP） content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame （ORF） of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-Ala and TaZds-Alb, yielding 183- and 179-bp fragments in TaZds-Ala and TaZds-Alb genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid （DH） population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-Alb allele was 7.8% higher than that of 91 cultivars with TaZds- Ala allele.ζ （zeta）-carotene desaturase （ZDS） is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment （YP） content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame （ORF） of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-Ala and TaZds-Alb, yielding 183- and 179-bp fragments in TaZds-Ala and TaZds-Alb genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid （DH） population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-Alb allele was 7.8% higher than that of 91 cultivars with TaZds- Ala allele.

关 键 词：Triticum aestivum functional marker QTL ζ-carotene desaturase ZDS 

分 类 号：S511[农业科学—作物学] S512.103