聚合酶链式反应检测银屑病患者咽部菌群中M6蛋白  

Detection of M6-protein on pharyngeal florae of patients with guttate psoriasis by polymerase chain reaction

作　　者：赵广[1] 冯晓玲[2] 江永强 蔡庆[3] 那爱华[1] 蔡瑞康[1] 杜孝元[3] 梅英杰[1] 

机构地区：[1]空军总医院皮肤科,北京市100036 [2]空军总医院感染科 [3]空军总医院临床实验科

出　　处：《临床皮肤科杂志》2000年第2期87-89,共3页Journal of Clinical Dermatology

摘　　要：根据编码M 6蛋白的emm6基因序列设计一对寡核苷酸引物 ,可特异扩增2 2 0bp的DNA片段。应用聚合酶链式反应(PCR)方法对点滴型银屑病患者咽部培养出的 19株细菌中的M 6蛋白基因进行检测 ,结果 ,15株链球菌中 6株为阳性 ,其中化脓链球菌 3株 ,无乳链球菌、似马链球菌和乳房链球菌各 1株 ,4株非链球菌均为阴性。M 6蛋白基因不仅存在化脓性溶血链球菌中 ,在其它链球菌如乳房链球菌中也存在。研究结果显示 ,化脓性溶血链球菌中的化脓链球菌、无乳链球菌和似马链球菌与点滴型银屑病的发生有明显相关性 ,可能主要是菌体中的M 6蛋白在起作用 ,其它含有M 6蛋白的细菌如乳房链球菌也可诱发或加重银屑病的发生。A pair of oligonucleotide primers derived form emm6, the gene encoding M6 protein, were chosen to specially amplify a 220bp DNA fragment The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1 S agalactiae, 1 S eguisimilis and 1 S uberis, and 4 non streptococus strains were negative Emm6 was found not only in suppurative hemolytic streptococus, but also in S uberis The results shows that suppurative hemolytic streptococus such as S pyogenes, S agalactiae and S eguisimilis are obviously related to guttate psoriasis, and M6 proteins plays an important role Other bacteria containing M6 protein liking S uberis can alsc induce or exaggerate the psoriasis

关 键 词：点滴型银屑病 聚合酶链反应 M6蛋白 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]