日本血吸虫病人群获得性免疫与再感染　Ⅲ.人外周血单核细胞培养时细胞因子的产生与对日本血吸虫感染的抗性关系研究  

ACQUIRED IMMUNITY AND REINFECTION TO SCHISTOSOMA JAPONICUM Ⅲ. STUDY ON ASSOCIATION BETWEEN CYTOKINE PRODUCTION BY PERIPHERAL BLOOD MONONUCLEAR CELLS IN VITRO AND RESISTANCE TO SCHISTOSOMA JAPONICUM INFECTION IN RESIDENTS FROM ENDEMIC AREA

作　　者：李毅[1] G.J.Waine 李岳生[1] A.G.P.Ross 张新跃[1] 黎红燕 A.C.Sleigh D.P.McManus 

机构地区：[1]湖南省寄生虫病防治研究所,岳阳414000 [2]澳大利亚昆士兰医学研究所

出　　处：《中国血吸虫病防治杂志》2000年第2期75-79,共5页Chinese Journal of Schistosomiasis Control

基　　金：TDR及澳大利亚国家医药卫生研究委员会资助项目

摘　　要：目的观察洞庭湖日本血吸虫病流行区居民（实验组）和非疫区居民（对照组）的外周血单核细胞（ＲＢＭＣ）在体外培养条件下，用日本血吸虫成虫可溶性抗原（ＡＷＡ）和５种重组基因疫苗候选抗原刺激增生时细胞因子的产生情况，并评价这些细胞因子的产生与人体对日本血吸虫感染抗性之间的关系。方法将来自洞庭湖日本血吸虫病洲岛型流行区２个岛（青山和严家山）的６７名实验组对象，按其暴露于疫水的程度和血吸虫病粪检结果，分为抗性组（有较高的暴露程度但血吸虫粪卵阴性）、易感组（血吸虫粪卵阳性）和不确定组（有较低程度的暴露且血吸虫粪卵阴性），来自非疫区的１１名居民作为对照组。将试验组和对照组对象的外周血单核细胞在血吸虫成虫可溶性抗原和５种重组抗原（副肌球蛋白、２８ ｋＤａ谷胱甘肽－Ｓ－转移酶、脂肪酸结合蛋白、２２ ｋＤａ表皮膜相关蛋白和 ３－磷酸甘油醛脱氢酶）的刺激下培养 ５ ｄ，用 ＥＬＩＳＡ法检测培养上清液中的白细胞介素 ５（ＩＬ－５），白细胞介素１０（ＩＬ－１０）和γ－干扰素（ＩＦＮ－γ）的含量。结果试验组对象的ＰＢＭＣ在ＡＷＡ刺激下产生的 ３种细胞因子的水平均高于对照组；但在重组抗原刺激下，只有 ＩＬ－１０的产生高于对照组；抗性组对象的 ＰＢＭＣ?Objectives To observer the cytokine profiles producted by human peripheral blood mononuclear cells (PBMC) in vitro following stimulation with S. japonicum soluble adult worm antigen (AWA) or five recombinant S. japonicum antigens, and to assess the association between these cytokine production and resistance to S. japonicum infection. Methods According to epidemiological data, 67 observational group people residing in an area endemic for schistosomiasis japonica (exposed group ) were further divided into three groups. The first was resistant group (with high exposure level and negative in stool examination for schistosome egg, n=21 ), the second was susceptible group (positive in stool examination, n=16) and the third was indeterminate group (with lower exposure level and negative in stool examination, n=30). Peripheral blood monomuclear cells from subjects of observational group and individuals residing in non-endemic area (control group) were incubated in the presence of AWA, or five recombinant antigens. Cytokine including interleukin-5 (IL-5), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in supernatants were determined quantitatively by sandwich-ELISA using cytokine-specific monoclonal antibodies. Results Following the stimulation with AWA, PBMCs from individuals of observational group produced significantly higher level of IL-5, IL-10 and IFN-γ when compared with those from the control. When PBMCs were incubated in the presence of one of five recombinant schistosome antigens, there was only significant difference in IL-10 production between observational and control groups. Following the stimulation of TegAg,FABP or GAPDH,PBMCs from individuals of resistant group produced markedly higher levels of IL-10 when compared with those from susceptible group. Following the stimulation of GST, level of IFN-γ in supernatants of PBMCs from resistant individuals were significantly higher than that of PBMCs from subjects of susceptible group. No direct evidence of relationship between cytokine production an

关 键 词：日本血吸虫病 免疫反应 细胞增殖 细胞因子 

分 类 号：R532.21[医药卫生—内科学]