血小板环氧化酶及P-选择素的检测及其在冠心病患者中的表达  

Determination of platelet cyclooxygenases and P-selectin in patients with coronary artery disease

作　　者：龚晓璇[1] 张锦英[2] 张秋[1] 朱甜甜[1] 王晓伟[3] 王学忠[4] 殷咏梅[2] 李春坚[1] 杨志健[1] 

机构地区：[1]南京医科大学第一附属医院心脏科,江苏南京210029 [2]南京医科大学第一附属医院肿瘤科,江苏南京210029 [3]南京医科大学第一附属医院胸外科,江苏南京210029 [4]安徽马鞍山市人民医院心脏科,安徽马鞍山214300

出　　处：《南京医科大学学报（自然科学版）》2012年第7期948-951,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81170181);江苏省人民医院创新团队基金(2011)

摘　　要：目的:建立用流式细胞术测定富血小板血浆(platelet-rich plasma,PRP)中血小板环氧化酶-1(cyclooxygenase-1,COX-1)和环氧化酶-2(cyclooxygenase-2,COX-2)及P-选择素表达的方法;检测在无抗血小板药物干预的状况下中国汉族冠心病患者血小板COX-1、COX-2及P-选择素的表达情况。方法:入选拟行冠状动脉搭桥术、停用阿司匹林及氯吡格雷7 d以上的冠心病患者20例,采集空腹静脉血,经预处理及荧光标记抗体孵育后用流式细胞仪检测血小板COX-1、COX-2及P-选择素的表达情况。结果:在无抗血小板药物干预的状况下,中国汉族冠心病患者血小板COX-1、COX-2阳性表达比例分别为(39.67±9.20)%和(4.11±2.74)%;生理盐水刺激后血小板膜表面P-选择素的阳性表达率为(13.56±8.07)%,花生四烯酸刺激后的阳性表达率为(24.08±13.49)%,血小板膜表面P-选择素的实际阳性表达率为(10.27±7.40)%。结论:流式细胞术可有效测定血小板COX-1、COX-2和P-选择素的表达,冠心病患者血小板COX-1、COX-2和P-选择素的表达在中国汉族人种与高加索人种间可能存在差异。Objective：To establish a method of detecting cyclooxygenase （COX）-I,COX-2 and P-selectin in platelet-rich plasma （PRP） by flow eytometry （FCM）,and to detect the expressions of COX,COX-2 and P-seleetin in PRP from coronary artery disease （CAD） patients without using antiplatelet agents. Methods：Twenty CAD patients were recruited, who were going to receive coronary artery bypass graft（CABG） and had withdrawn aspirin and clopidogrel for at least 7 days. After overnight fasting, the patients＇ venous blood was drawn and processed,then the PRP was incubated with fluorescence labeled antibody, and COX-l, COX-2 and P-seleetin were detected by FCM. Results：The positive percent of COX-l, COX-2 in PRP of the enrolled 20 patients were （39.67 ±9.20）% and （4.11 ± 2.74）%,respectively,and the positive percent of P-selectin in platelet was （13.56 ± 8.07）% after incubated with saline and （24.08±13.49）% after incubated with araehidonic acid,the real positive percent of P-seleetin in platelet was （10.27 ± 7.40）%. Conclusion： FCM can effectively detect the platelet expressions of COX-l, COX-2 and P-seleetin. There would be ethnic differences between Chinese Han and Caucasian regarding the platelet expressions of COX-l, COX-2 and P-seleetin in CAD patients.

关 键 词：环氧化酶-1 环氧化酶-2 P-选择素 流式细胞术 冠心病 

分 类 号：R541.4[医药卫生—心血管疾病]