Preparation of hemicellulolic oligosaccharides from Chamaecyparis obtuse （Hinoki） slurry using commercial enzymes  

作　　者：Yuya KUMAGAI Hirokazu USUKI Yukihiro YAMAMOTO Akihiro YAMASATO Takafumi MUKAIHARA Tadashi HATANAKA 

机构地区：[1]Okayama Prefectural Technology Center for Agriculture,Forestry and Fisheries,Research Institute for Biological Sciences(RIBS),Okayama 716-1241,Japan [2]Research Fellow of the Japan Society for the Promotion of Science(JSPS),Japan [3]Nagoya University,Nagoya 464-8601,Japan

出　　处：《Frontiers of Chemical Science and Engineering》2012年第2期224-231,共8页化学科学与工程前沿（英文版）

摘　　要：Wood biomass is anticipated to serve as a substitute for carbon source, which has no feedstock competition with foods. Biomass is commonly used for the production of bio-ethanol by a series of processes such as pretreatment, enzymatic degradation, and fermentation. Hemicellulose, constituting 20 wt-% - 40 wt-% ofbiomass materials, contains various kinds of saccharides known to be bioactive substrates. Practical usage of hemicellulose is generally limited to its conversion to bio-ethanol. Here, we aimed to prepare hemicellulolic oligosaccharides, more valuable products other than ethanol. Therefore, the Hinoki slurry was treated with lime at room temperature for 3 h, and then neutralized with HCI. The resulting sample was treated with 13 types of commercial enzymes, and the saccharides produced in the supernatant were evaluated. The result showed that the commercial enzyme Cellulase SS （Nagase ＆ Co., LTD.） effectively degraded the slurry to produce disaccharides and trisaccharides. Analysis of sugar components by liquid chromatography/mass spectrography （LC/MS） after the derivation with ethyl 4- aminobenzoate （ABEE） showed that mannobiose, manno- Nose, and cellobiose were the major oligosaccharides. These results indicate valuable oligosaccharides can be successfully produced from Hinoki softwood slurry.Wood biomass is anticipated to serve as a substitute for carbon source, which has no feedstock competition with foods. Biomass is commonly used for the production of bio-ethanol by a series of processes such as pretreatment, enzymatic degradation, and fermentation. Hemicellulose, constituting 20 wt-% - 40 wt-% ofbiomass materials, contains various kinds of saccharides known to be bioactive substrates. Practical usage of hemicellulose is generally limited to its conversion to bio-ethanol. Here, we aimed to prepare hemicellulolic oligosaccharides, more valuable products other than ethanol. Therefore, the Hinoki slurry was treated with lime at room temperature for 3 h, and then neutralized with HCI. The resulting sample was treated with 13 types of commercial enzymes, and the saccharides produced in the supernatant were evaluated. The result showed that the commercial enzyme Cellulase SS （Nagase ＆ Co., LTD.） effectively degraded the slurry to produce disaccharides and trisaccharides. Analysis of sugar components by liquid chromatography/mass spectrography （LC/MS） after the derivation with ethyl 4- aminobenzoate （ABEE） showed that mannobiose, manno- Nose, and cellobiose were the major oligosaccharides. These results indicate valuable oligosaccharides can be successfully produced from Hinoki softwood slurry.

关 键 词：hemicellulolic oligosaccharides Chamaecyparis obtuse （Hinoki） commercial enzyme manno-oligosaccharide cello-oligosaccharide 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] Q533[轻工技术与工程—食品科学与工程]