四氯化碳造成急性肝损伤大鼠模型的实验研究  

CCl4 causing acute liver injury in a rat model

作　　者：刘赴平[1,2] 师玲玲[2] 

机构地区：[1]暨南大学第一临床医学院,广东广州510632 [2]东莞市中心血站

出　　处：《中国输血杂志》2012年第5期446-448,共3页Chinese Journal of Blood Transfusion

基　　金：东莞市科技立项(2008105150148)

摘　　要：目的评估建立四氯化碳(CCl4)造成大鼠急性肝损伤模型的效果。方法健康SPF级SD大鼠40只,随机分为模型组(n=30):腹腔注射50%CCl4橄榄油溶液3 ml/kg.只;对照组(n=10)腹腔注射生理盐水溶液3ml/kg.只。比较造模后24、72 h、1周3个时间点模型鼠外周血AST/ALT水平及造模后24、48 h模型鼠存活率,观察造模后24 h模型鼠肝脏组织病理改变。结果造模后24、72、1周3个时间点急性肝损伤模型鼠ALT/AST水平为(198.45±15.02)/(498.2±29.83)、(154.27±11.82)/(373.58±20.45)、(88.4±6.8)/(224.7±29.55),明显高于对照组相应时间的ALT/AST(P<0.01),造模后24 h和48 h模型组实验动物存活率分别为40%和10%,对照组实验动物存活率均为100%(P<0.01),病理切片显示模型鼠肝脏呈急性肝坏死表现。结论所构建的CCl4造成大鼠急性肝坏死造模体系适宜实验需求。Objective To evaluate the acute liver injury rat model.Methods 40 SD rats were randomly divided into two groups： 10 rats for control group,30 rats for model group.50% CCl4 olive oil solution（3 ml/kg） was injected intraabdominally for model group rats,saline solution（3 ml/kg） was injected into control rats.AST / ALT levels were compared between the two groups at 24 h,72 h,and one week after the injection of CCl4 or normal saline.The survival rates of two groups at 24 h,48 h post-model were analyzed.The pathological changes of liver at 24 h post-model were observed.Results The ALT / AST level difference between model group and control group was statistically significant（P〈0.01） at all time points.The survival rate were also statistically significant different between the 2 groups（P〈0.01） at 24 h,and 48 h post-model.Liver sections revealed acute hepatic necrosis in model group.Conclusion The acute hepatic necrosis model is suitable for further experimental needs.

关 键 词：肝损伤 四氯化碳 大鼠模型 ALT AST 

分 类 号：R457.1[医药卫生—治疗学] R446.112[医药卫生—临床医学]