万寿菊杀菌素Ⅰ抗菌性及其对西瓜枯萎病菌作用机理的初步研究  

Antifungal activity of marigold fungicide I and its mechanism on Fusarium oxysporum f.sp.niveum

作　　者：范志宏[1] 郭春绒[1] 王金胜[2] 

机构地区：[1]山西农业大学文理学院,太谷030801 [2]山西农业大学生命科学学院,太谷030801

出　　处：《植物病理学报》2012年第4期445-448,共4页Acta Phytopathologica Sinica

基　　金：山西省科技攻关项目(20090311028);山西农业大学科技创新基金项目(2006021)

摘　　要：西瓜枯萎病又称萎蔫病或蔓割病，是一种世界性瓜类土传病害。自1894年在美国南卡罗来纳州首次发现以来，目前几乎发生于世界各个西瓜产区，严重威胁着西瓜的安全生产，是西瓜的主要病害之一。近几年来，由于西瓜种植面积不断扩大，造成轮作困难，致使枯萎病的危害也逐年加重。研究表明，万寿菊根的提取物对西瓜枯萎病菌有明显的抑制作用，能诱导西瓜保护酶活性提高而减轻西瓜枯萎病菌对西瓜幼苗的毒害作用，Marigold fungicide I was studied about the antifungal activity on several pathogenic fungi and the mechanism against Fusarium oxysporum f. sp. niveum （ FON）, synthetic analogue of extracts of Tagetes pat- ula root. The results showed that marigold fungicide I remarkably inhibited the mycelial growth of several pathogenic fungi, which possessed the content and time effects on Fusarium oxysporum schlecht, f. sp. niveum, Phytophthpra capsici Loen, Botrytis cinerea Pers. and Fulvia fulva （Cookee） Ciferri, threshold effects on Fu- sarium oxysporum f. sp. capsici and Gibberella zeae（ Schw. ）Petch and content effects on Glomerella gossypii （Southw.） Edgertin. Marigold fungicide I was applied on FON and manifested the following findings： reduced the dry weight of mycelium, amplified membrane permeability, shortly increased chitinase activity, but no change of POD isozyme. The electrophoresis of total protein by SDS- PAGE showed that marigold fungi- cide I apparently affected the species and expression amounts of protein of FON.

关 键 词：西瓜枯萎病菌 万寿菊 抗菌性 杀菌素 机理 南卡罗来纳州 土传病害 保护酶活性 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]