刺糖多孢菌生长特性及移种标准  

Growth Characteristic and Inoculation Standard for Saccharopolyspora spinosa

作　　者：王子宝[1] 沈淑媛[1] 裘娟萍[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《农药》2012年第7期494-498,共5页Agrochemicals

基　　金：浙江省农业科技重大专项重点项目(2009C12062)

摘　　要：[目的]掌握刺糖多孢菌生长代谢特性,为提高多杀菌素产量及发酵稳定性提供依据。[方法]对刺糖多孢菌斜面及种子生长规律、培养工艺及移种标准进行研究。[结果]确定斜面移种标准为平板单菌落移种第1代斜面,菌龄7 d;液体种子最佳培养工艺为添加30粒玻璃珠,温度30℃,初始pH值8.5;液体种子移种标准为生物量大于1.4 g/L、氨基氮75～80 mg/L、还原糖低于7 g/L、pH值低于7.5、种龄30～36 h。[结论]采用标准种子移种,多杀菌素产量提高58.5%,不同批次间产量差异小于5%。[Aims] The aim is to investigate the growth metabolism characteristic of Saccharopolyspora spinosa and to provide the basic materials for increasing spinosad production and fermentation stability. [Methods] The growth rule, cultivation process and inoculation standard of Saccharopolyspora spinosa were studied. [Results] The result showed that the cultivation of single colony on the plate for 7 days was the best inoculation standard of the slant strains. The optimized culture conditions of liquid seed were established as a combination of the glass bead of 30 grains in 500 mL shake flask, cultural temperature at 30 ~C and initial pH of 8.5. The inoculation standard of liquid seed consisted of biomass （greater than 1.4 g/L）, amino nitrogen （75-80 mg/L）, of reduced sugar （less than 7 g/L）, pH （less than 7.5） and inoculum age （30- 36 h）. [Conclusions] Employing the new inoculation standard, the spinosad production is increased by 58.5% and the difference of spinosad production among different batches is less than 5%.

关 键 词：刺糖多孢菌 多杀菌素 生长特性 移种标准 

分 类 号：TQ450.1[化学工程—农药化工]